{Reference Type}: Journal Article
{Author}: Dey, Biswajit; Bhunia, Sanjoy K.; Maity, Kankan K.; Patra, Sukesh; Mandal, Soumitra; Behera, Birendra; Maiti, Tapas K.; Sikdar, Samir R.; Islam, Syed S.
{Year}: 2013
{Title}: Structure and immunology of a heteroglycan from somatic hybrid mushroom of Pleurotus florida and Calocybe indica var. APK2
{Tag}: 5
{Star}: 0
{Journal}: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
{Volume}: 52
{Pages}: 312-318
{ISBN/ISSN}: 0141-8130
{Keywords}: EDIBLE MUSHROOM; CHEMICAL-ANALYSIS; ALKALINE EXTRACT; POLYSACCHARIDES; GLUCAN; (1->3)-BETA-D-GLUCAN; MACROPHAGES; ANTITUMOR; FRACTION; BODIES; Somatic hybrid PCH3FB; Pleurotus florida; Calocybe indica var. APK2; Mushroom polysaccharide; NMR spectroscopy; Immunoactivation
{Abstract}: A water soluble polysaccharide was isolated from the aqueous extract of the fruit bodies of somatic hybrid mushroom PCH3FB, obtained through protoplast fusion between the strains Pleurotus florida and Calocybe indica var. APK2. On the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies (H-1, C-13, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC), the structure of the repeating unit of the polysaccharide was established as:
[GRAPHICS]
This molecule showed macrophage, splenocyte and thymocyte activations. (C) 2012 Elsevier B.V. All rights reserved.
{Author Address}: Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Indian Inst Technol IIT Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India; Indian Inst Technol IIT Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India; Bose Inst, Div Plant Biol, Calcutta 700054, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India; India; India

{Reference Type}: Journal Article
{Author}: Gil-Ramirez, Alicia; Clavijo, Cristina; Palanisamy, Marimuthu; Ruiz-Rodriguez, Alejandro; Navarro-Rubio, Maria; Perez, Margarita; Marin, Francisco R.; Reglero, Guillermo; Soler-Rivas, Cristina
{Year}: 2013
{Title}: Screening of edible mushrooms and extraction by pressurized water (PWE) of 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitors
{Tag}: 5
{Star}: 0
{Journal}: JOURNAL OF FUNCTIONAL FOODS
{Volume}: 5
{Issue}: 1
{Pages}: 244-250
{ISBN/ISSN}: 1756-4646
{Keywords}: HYPERCHOLESTEROLEMIC RATS; LENTINUS-EDODES; POLYSACCHARIDES; ERITADENINE; FOOD; Cholesterol; HMGCoA reductase; Pleurotus ostreatus; Lentinus edodes; Accelerated solvent extraction (ASE)
{Abstract}: The methanol/water and particularly the water extracts obtained from 26 mushroom species were able to inhibit the 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGCR) activity to different extent (10-76%). Cultivated mushrooms such as Pleurotus sp. and Lentinula edodes were among the strains which showed higher HMGCR inhibitory capacities. Their inhibitory properties were not largely influenced by cultivation parameters, mushroom developmental stage or flush number. The HMGCR inhibitory activity of L. edodes was concentrated in the cap excluding the gills while in Pleurotus ostreatus it was distributed through all the different tissues. A method to obtain aqueous fractions with high HMGCR inhibitory activity was optimized using an accelerated solvent extractor (ASE) by selecting 10.7 MPa and 25 degrees C as common extraction conditions and 5 cycles of 5 min each for P. ostreatus fruiting bodies and 15 cycles of 5 min for L. edodes suggesting that the potential HMGCR inhibitors are different in the two selected mushrooms. (C) 2012 Elsevier Ltd. All rights reserved.
{Author Address}: UAM CSIC, CIAL Res Inst Food Sci, Dept Prod & Characterizat New Foods, Madrid 28049, Spain; Ctr Tecnol Invest Champinon La Rioja CTICH, Autol, Spain; UAM CSIC, CIAL Res Inst Food Sci, Dept Prod & Characterizat New Foods, Madrid 28049, Spain; UAM CSIC, CIAL Res Inst Food Sci, Dept Prod & Characterizat New Foods, Madrid 28049, Spain; UAM CSIC, CIAL Res Inst Food Sci, Dept Prod & Characterizat New Foods, Madrid 28049, Spain; Ctr Tecnol Invest Champinon La Rioja CTICH, Autol, Spain; UAM CSIC, CIAL Res Inst Food Sci, Dept Prod & Characterizat New Foods, Madrid 28049, Spain; UAM CSIC, CIAL Res Inst Food Sci, Dept Prod & Characterizat New Foods, Madrid 28049, Spain; UAM CSIC, CIAL Res Inst Food Sci, Dept Prod & Characterizat New Foods, Madrid 28049, Spain
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Spain; Spain

{Reference Type}: Journal Article
{Author}: Finimundy, T. C.; Gambato, G.; Fontana, R.; Camassola, M.; Salvador, M.; Moura, S.; Hess, J.; Henriques, J. A. P.; Dillon, A. J. P.; Roesch-Ely, M.
{Year}: 2013
{Title}: Aqueous extracts of Lentinula edodes and Pleurotus sajor-caju exhibit high antioxidant capability and promising in vitro antitumor activity
{Tag}: 0
{Star}: 0
{Journal}: NUTRITION RESEARCH
{Volume}: 33
{Issue}: 1
{Pages}: 76-84
{ISBN/ISSN}: 0271-5317
{Keywords}: WATER-SOLUBLE POLYSACCHARIDE; TUMOR-CELL-LINES; EDIBLE MUSHROOM; STRUCTURAL-ANALYSIS; SHIITAKE MUSHROOMS; CULTURE-MEDIUM; FRACTION; MYCELIA; SCHIZOPHYLLAN; FEASIBILITY; Lentinula edodes; Pleurotus sajor-caju; Hep-2; HeLa; Tumor cell lines
{Abstract}: Mushroom extracts are increasingly sold as dietary supplements because of several of their properties, including the enhancement of immune function and antitumor activity. We hypothesized that soluble polar substances present in mushroom extracts may show antioxidant and anticancer properties. This report shows that Brazilian aqueous extracts of Lentinula edodes and Pleurotus sajor-caju exert inhibitory activity against the proliferation of the human tumor cell lines laryngeal carcinoma (Hep-2) and cervical adenocarcinoma (HeLa). Cell viability was determined after using 3 different temperatures (4 degrees C, 22 degrees C, and 50 degrees C) for mushroom extraction. Biochemical assays carried out in parallel indicated higher amounts of polyphenols in the L edodes extracts at all extraction temperatures investigated. The scavenging ability of the 2,2-diphenyl-1-picrylhydrazyl radical showed higher activity for L edodes extracts. Superoxide dismutase like activity showed no statistically significant difference among the groups for the 2 tested extracts, and catalase-like activity was increased with the L edodes extracts at 4 degrees C. The results for the cytotoxic activity from P sajor-caju extracts at 22 degrees C revealed the half maximal inhibitory concentration values of 0.64% +/- 0.02% for Hep-2 and 0.25% +/- 0.02% for HeLa. A higher cytotoxic activity was found for the L edodes extract at 22 degrees C, with half maximal inhibitory concentration values of 0.78% +/- 0.02% for Hep-2 and 0.57% +/- 0.01% for HeLa. Substantial morphological modifications in cells were confirmed by Giemsa staining after treatment with either extract, suggesting inhibition of proliferation and induction of apoptosis with increasing extract concentrations. These results indicate that the aqueous extracts of Brazilian L edodes and P sajor-caju mushrooms are potential sources of antioxidant and anticancer compounds. However, further investigations are needed to exploit their valuable therapeutic uses and to elucidate their modes of action. (C) 2013 Elsevier Inc. All rights reserved.
{Author Address}: Univ Caxias Sul, Lab Genom Prote & DNA Repair, BR-95070560 Caxias Do Sul, RS, Brazil; Univ Caxias Sul, Lab Genom Prote & DNA Repair, BR-95070560 Caxias Do Sul, RS, Brazil; Univ Caxias Sul, Lab Enzyme & Biomass, BR-95070560 Caxias Do Sul, RS, Brazil; Univ Caxias Sul, Lab Enzyme & Biomass, BR-95070560 Caxias Do Sul, RS, Brazil; Univ Caxias Sul, Lab Oxidat Stress & Antioxidants, BR-95070560 Caxias Do Sul, RS, Brazil; Univ Caxias Sul, Lab Essential Oils, Inst Biotechnol, BR-95070560 Caxias Do Sul, RS, Brazil; Univ Heidelberg Hosp, Dept Otolaryngol Head & Neck Surg, D-69120 Heidelberg, Germany; German Canc Res Ctr, DKFZ ZMBH Alliance, Jr Res Grp Mol Mech Head & Neck Tumors A102, D-69120 Heidelberg, Germany; Univ Caxias Sul, Lab Genom Prote & DNA Repair, BR-95070560 Caxias Do Sul, RS, Brazil; Univ Caxias Sul, Lab Enzyme & Biomass, BR-95070560 Caxias Do Sul, RS, Brazil; Univ Caxias Sul, Lab Genom Prote & DNA Repair, BR-95070560 Caxias Do Sul, RS, Brazil
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Brazil; Brazil; Brazil; Brazil; Germany; Germany

{Reference Type}: Journal Article
{Author}: Maji, Praloy K.; Sen, Ipsita K.; Devi, K. Sanjana P.; Maiti, Tapas K.; Sikdar, Samir R.; Islam, Syed S.
{Year}: 2013
{Title}: Structural elucidation of a biologically active heteroglycan isolated from a hybrid mushroom of Pleurotus florida and Lentinula edodes
{Tag}: 0
{Star}: 0
{Journal}: CARBOHYDRATE RESEARCH
{Volume}: 368
{Pages}: 22-28
{ISBN/ISSN}: 0008-6215
{Keywords}: EDIBLE-MUSHROOM; AQUEOUS EXTRACT; BRANCHED (1->3)-BETA-D-GLUCAN; IMMUNOENHANCING PROPERTIES; VOLVARIELLA-VOLVACEA; ANTITUMOR-ACTIVITY; ALKALINE EXTRACT; POLYSACCHARIDE; FUNGI; PURIFICATION; Hybrid mushroom; Heteroglycan; NMR studies; Immunoactivation
{Abstract}: A water soluble heteroglycan isolated from hot aqueous extract of fruit bodies of an edible hybrid mushroom Pfle1r of Pleurotus florida and Lentinula edodes consists of D-glucose, D-mannose, and D-galactose residues in a molar ratio of nearly 1:1:1. This polysaccharide showed macrophage, splenocyte, and thymocyte activation. On the basis of sugar hydrolysis, methylation, periodate oxidation study, and NMR studies (H-1, C-13, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as:
[GRAPHICS]
.
(C) 2012 Elsevier Ltd. All rights reserved.
{Author Address}: Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India; Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India; Bose Inst, Div Plant Biol, Calcutta 700054, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India; India; India