{Reference Type}: Journal Article
{Author}: Sen, Ipsita K.; Maji, Praloy K.; Behera, Birendra; Maiti, Tapas K.; Mallick, Pijush; Sikdar, Samir R.; Islam, Syed S.
{Year}: 2013
{Title}: Glucan of a somatic hybrid mushroom, pfls 1h: structural characterization and study of immunological activities
{Tag}: 5
{Star}: 0
{Journal}: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
{Volume}: 53
{Pages}: 127-132
{ISBN/ISSN}: 0141-8130
{Keywords}: SQUARROSULUS MONT. SINGER; PLEUROTUS-FLORIDA; EDIBLE MUSHROOM; ALKALINE EXTRACT; CHEMICAL-ANALYSIS; AQUEOUS EXTRACT; POLYSACCHARIDE; ANTITUMOR; INDUCTION; OSTREATUS; Polysaccharide; NMR; Splenocytes and thymocytes; Macrophages
{Abstract}: A water-soluble glucan (PS-I) was isolated from the aqueous extract of the fruit bodies of a hybrid mushroom, pfls 1h of Pleurotus florida and Lentinus squarrosulus (Mont.) Singer. Structural characterization of PS-I was carried out using total hydrolysis, methylation analysis, periodate oxidation, and NMR experiments (H-1, C-13, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC). Methylation analysis revealed that PS-I was composed of (1 -> 3, 6), (1 -> 3), (1 -> 6)-linked and terminal beta-D-glucopyranosyl residues in a relative proportion of approximately 1:1:1:1. The repeating unit of the glucan consists of a backbone chain of two (1 -> 6)-beta-D-glucopyranosyl residues, one of which is branched at O-3 position with (1 -> 3)-beta-D-glucopyranosyl and terminated with a beta-D-glucopyranosyl residue. Study of immunological activity revealed that PS-I stimulates the splenocytes, thymocytes and macrophages. (c) 2012 Elsevier B.V. All rights reserved.
{Author Address}: Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Indian Inst Technol IIT Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India; Indian Inst Technol IIT Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India; Bose Inst, Div Plant Biol, Calcutta 700054, W Bengal, India; Bose Inst, Div Plant Biol, Calcutta 700054, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India; India; India

{Reference Type}: Journal Article
{Author}: Patra, Sukesh; Patra, Pradip; Maity, Kankan K.; Mandal, Soumitra; Bhunia, Sanjay K.; Dey, Biswajit; Devi, K. Sanjana P.; Khatua, Somanjana; Acharya, Krishnendu; Maiti, Tapas K.; Islam, Syed S.
{Year}: 2013
{Title}: A heteroglycan from the mycelia of Pleurotus ostreatus: structure determination and study of antioxidant properties
{Tag}: 0
{Star}: 0
{Journal}: CARBOHYDRATE RESEARCH
{Volume}: 368
{Pages}: 16-21
{ISBN/ISSN}: 0008-6215
{Keywords}: OYSTER MUSHROOM; ANTITUMOR POLYSACCHARIDES; EDIBLE MUSHROOM; ANTI-GLYCATION; EXTRACT; CULTIVATION; FUNGI; RATS; RIND; Pleurotus ostreatus; Mycelia; Heteroglycan; Structure; NMR studies; Antioxidant properties
{Abstract}: A heteroglycan (PS) isolated from the mycelia of Pleurotus ostreatus was found to consist of L-fucose, D-mannose, and D-glucose in a molar ratio of nearly 1:2:3. On the basis of acid hydrolysis, methylation analysis, periodate oxidation, and NMR studies (H-1, C-13, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, HMBC, and DEPT), the structure of the repeating unit of the PS was determined. The repeating unit had a branched backbone composed of (1 -> 6)-linked alpha and beta D-glucose and (1 -> 2)-linked alpha-L-fucose. Branching occurred at C-4 position of (1 -> 6)-linked alpha-D-glucopyranosyl residue with terminal beta-D-mannose and C-3 position of (1 -> 6)-linked beta-D-glucopyranosyl residue with (1 -> 6)-alpha-D-mannopyranosyl moiety terminated by alpha-D-glucose. The structure of the repeating unit of the PS was proposed as:
[GRAPHICS]
The antioxidant properties of the PS were studied. The scavenging activity of hydroxyl free radicals whose EC50 value of PS at 943 mu g/mL was determined. Further, EC50 value of the PS at 53 mu g/mL was observed for scavenging activity of superoxide free radicals. Chelating effects (54.82%) of ferrous ions were observed at 1 mg/mL of the PS. (C) 2013 Elsevier Ltd. All rights reserved.
{Author Address}: Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India; Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India; Univ Calcutta, Dept Bot, Mol & Appl Mycol & Plant Pathol Lab, Calcutta 700019, W Bengal, India; Univ Calcutta, Dept Bot, Mol & Appl Mycol & Plant Pathol Lab, Calcutta 700019, W Bengal, India; Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India; Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India; India; India

{Reference Type}: Journal Article
{Author}: He, Peixin; Geng, Lujing; Mao, Duobin; Wang, Zheng; Xu, Chunping
{Year}: 2013
{Title}: PRODUCTION, PRELIMINARY CHARACTERIZATION, AND BIOACTIVITIES OF EXOPOLYSACCHARIDES FROM Pleurotus geesteranus 5 #
{Tag}: 0
{Star}: 0
{Journal}: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
{Volume}: 43
{Issue}: 1
{Pages}: 108-122
{ISBN/ISSN}: 1082-6068
{Keywords}: POLYSACCHARIDE-PROTEIN COMPLEXES; WATER-SOLUBLE POLYSACCHARIDES; SUBMERGED CULTURE-CONDITIONS; EXO-BIOPOLYMER PRODUCTION; ANTIOXIDANT ACTIVITIES; DIFFERENT FRACTIONS; ANTITUMOR-ACTIVITY; LIGHT-SCATTERING; TUBER-REGIUM; OPTIMIZATION; antioxidant; chromatography; exopolysaccharides; fermentation; Pleurotus geesteranus; purification; taguchi method
{Abstract}: The optimal culture conditions of exopolysaccharides (EPS) production in submerged culture medium by Pleurotus geesteranus 5 # were determined using an orthogonal matrix method. The optimal defined medium (per liter) was 60.0 g maltose, 5.0 g tryptone, 1 mM NaCl, 5 mM KH2PO4, and initial pH 6.0 at 28 degrees C. In the optimal culture medium, the maximum EPS production was 16.97 g/L in a shake flask. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography (SEC), and their molecular characteristics were examined by a multiangle laser-light scattering (MALLS) and refractive index (RI) detector system. The approximate weight-average molar masses of the Fr-I and Fr-II of EPS were determined to be 3.263 x 104 and 5.738 x 103 g/mol, respectively. The low values of polydispersity ratio (1.176 and 1.124 for Fr-I and Fr-II, respectively) of EPSs mean that these EPS molecules exist much less dispersed in aqueous solution without forming large aggregates. Furthermore, the experiments in vitro indicated that P. geesteranus 5# EPS exhibit high antitumor and antioxidative effects.
{Author Address}: Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Henan, Peoples R China; Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Henan, Peoples R China; Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Henan, Peoples R China; Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Henan, Peoples R China; Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Henan, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China

{Reference Type}: Journal Article
{Author}: Zhang, An-qiang; Xu, Mei; Fu, Li; Sun, Pei-long
{Year}: 2013
{Title}: Structural elucidation of a novel mannogalactan isolated from the fruiting bodies of Pleurotus geesteranus
{Tag}: 0
{Star}: 0
{Journal}: CARBOHYDRATE POLYMERS
{Volume}: 92
{Issue}: 1
{Pages}: 236-240
{ISBN/ISSN}: 0144-8617
{Keywords}: 3-O-METHYLATED MANNOGALACTAN; EDIBLE MUSHROOM; BETA-GLUCAN; POLYSACCHARIDE; OSTREATOROSEUS; SUGARS; Pleurotus geesteranus; Polysaccharide; Structural elucidation; NMR spectroscopy
{Abstract}: A novel water-soluble polysaccharide named PGPB-1, with a molecular weight of 1.3 x 10(4) Da as determined by high-performance liquid chromatography (HPLC), was isolated from the fruiting bodies of Pleurotus geesteranus by boiling water as well as DEAE-Sepharose Fast Flow and High-Resolution Sephacryl S-300. On the bases of compositional analysis, methylation analysis and NMR spectroscopy (H-1 NMR, C-13 NMR, 2D-COSY, TOCSY, HMQC, HMBC and NOESY spectra) analysis, the structural elucidation of PGPB-1 consisted of a alpha-D-(1 -> 6)-galactopyranan and a alpha-D-3-O-Me-D-galactosyl unit backbone with a alpha-D-Mannosyl unit on O-2 of the 2.6-di-O-substituted-D-galactosyl units. The repeating unit of the polysaccharide was established as:
[GRAPHICS]
PGPB-1 also contains a minor proportion of 6-linked-D-Glcp. (C) 2012 Elsevier Ltd. All rights reserved.
{Author Address}: Zhejiang Univ Technol, Coll Biol & Environm Engn, Hangzhou 310014, Zhejiang, Peoples R China; Zhejiang Univ Technol, Coll Biol & Environm Engn, Hangzhou 310014, Zhejiang, Peoples R China; Zhejiang Univ Technol, Coll Biol & Environm Engn, Hangzhou 310014, Zhejiang, Peoples R China; Zhejiang Univ Technol, Coll Biol & Environm Engn, Hangzhou 310014, Zhejiang, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China