{Reference Type}: Journal Article
{Author}: Synytsya, Andriy; Novak, Miroslav
{Year}: 2013
{Title}: Structural diversity of fungal glucans
{Tag}: 0
{Star}: 0
{Journal}: CARBOHYDRATE POLYMERS
{Volume}: 92
{Issue}: 1
{Pages}: 792-809
{ISBN/ISSN}: 0144-8617
{Keywords}: WATER-SOLUBLE GLUCAN; BETA-D-GLUCAN; PORIA-COCOS MYCELIA; PLEUROTUS-TUBER-REGIUM; MAGNETIC-RESONANCE SPECTROSCOPY; MUSHROOM CORIOLUS-VERSICOLOR; ANTI-TUMOR POLYSACCHARIDE; BLAZEI FRUITING BODIES; C-13 CHEMICAL-SHIFTS; ALPHA-D-GLUCAN; Fungal glucans; Structural diversity; Nuclear magnetic resonance; Chemical modification; Structure-activity relationship
{Abstract}: Fungal glucans represent various structurally different D-glucose polymers with a large diversity of molecular mass and configuration. According to glucose anomeric structure, it is possible to distinguish alpha-D-glucans, beta-D-glucans and mixed alpha,beta-D-glucans. Further discrimination could be made on the basis of glycosidic bond position in a pyranoid ring, distribution of specific glycosidic bonds along a chain, branching and molecular mass. Fungal glucans can be chemically modified to obtain various derivatives of potential industrial or medicinal importance. NMR spectroscopy is a powerful tool in structural analysis of fungal glucans. Together with chemolytic methods like methylation analysis and periodate oxidation. NMR is able to determine exact structure of these polysaccharides. Fungal glucans or their derivatives exert various biological activities, which are usually linked to structure, molecular mass and substitution degree. (C) 2012 Elsevier Ltd. All rights reserved.
{Author Address}: Prague Inst Chem Technol, Dept Carbohydrates & Cereals, CR-16628 Prague 6, Czech Republic; Prague Inst Chem Technol, Dept Carbohydrates & Cereals, CR-16628 Prague 6, Czech Republic
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Czech Republic

{Reference Type}: Journal Article
{Author}: Chen, Hua-Bing; Chen, Chih-I; Chen, Mei-Jheng; Lin, Chia-Chi; Kan, Shu-Chen; Zang, Chi-Zong; Yeh, Chiung-Wen; Shieh, Chwen-Jen; Liu, Yung-Chuan
{Year}: 2013
{Title}: The use of mushroom hydrolysate from waste bag-log as the nitrogen source to mycelium biomass and exopolysaccharide production in Pleurotus eryngii cultivation
{URL}: http://www.sciencedirect.com/science/article/pii/S1876107012001769
{Tag}: 0
{Star}: 0
{Journal}: Journal of the Taiwan Institute of Chemical Engineers
{Volume}: 44
{Issue}: 2
{Pages}: 163-168
{Date Displayed}: 2013/3//
{Alternate Title}: Journal of the Taiwan Institute of Chemical Engineers
{ISBN/ISSN}: 1876-1070
{Keywords}: Pleurotus eryngii; Mushroom hydrolysate; Waste treatment; Fermentation; Submerged culture; Microbial growth
{Abstract}: In this study, Pleurotus eryngii head part waste in a bag-log was collected and processed to yield mushroom hydrolysate powder (MHP). To test its stimulatory effect, a P. eryngii submerged culture was conducted. The prepared MHP (1.6%) and glucose (2%) were found to be the best sources for both mycelial biomass and EPS productions. A specific growth rate of 0.68 day−1, and a specific EPS yield of 9.55 g/g-dry-cell-weight were obtained under these conditions. It was also found that MHP gave higher mycelial biomass growth rate (2.05 g/L/day), and EPS yield (92 mg/L) than those of yeast extract. In 5-L fermentation with MHP as nitrogen source, high mycelial biomass growth rate (2.44 g/L/day) and EPS yield (312 mg/L) were obtained. In the solid state culture, P. eryngii hyphae growth rate in MHP agar (6.43 ± 0.21 mm/day) was better than that in PDA (4.57 ± 0.23 mm/day). By using PCA and cluster analysis, the major amino acids of MHP displayed a close similarity to those of P. eryngii mycelial hydrolysate powder (PEMHP).

{Reference Type}: Journal Article
{Author}: Zhang, An-qiang; Zhang, Yang; Yang, Jun-hong; Sun, Pei-long
{Year}: 2013
{Title}: Structural elucidation of a novel heteropolysaccharide from the fruiting bodies of Pleurotus eryngii
{URL}: http://www.sciencedirect.com/science/article/pii/S014486171201185X
{Tag}: 0
{Star}: 0
{Journal}: Carbohydrate Polymers
{Volume}: 92
{Issue}: 2
{Pages}: 2239-2244
{Date Displayed}: 2013/2/15/
{Alternate Title}: Carbohydrate Polymers
{ISBN/ISSN}: 0144-8617
{Keywords}: Pleurotus eryngii; Heteropolysaccharide; Structural elucidation; NMR analysis
{Abstract}: A novel water-soluble heteropolysaccharide (PEPS1), with a molecular weight of 1.88 × 104 Da as determined by high performance liquid chromatography (HPLC), specifically size exclusion chromatography, was isolated from the fruiting bodies of Pleurotus eryngii by hot water extraction and further purification by DEAE Sepharose Fast Flow and Sephacryl S-300 and S-100 High-Resolution chromatography. By use of compositional analysis, methylation analysis, together with 1H, 13C NMR and 2D NMR spectroscopy including COSY, TOCSY, HMQC, HMBC and NOESY experiments, the PEPS1 was identified as a heteropolysaccharide that consists of a α-d-(1 → 6)-Galactopyranan backbone with a β-d-Mannosyl unit on O-2 of the 2,6-di-O-substituted-d-Galactosyl units, α-(1 → 6)-3-O-Me-d-Galactopyranan backbone with a terminal α-3-O-Me-d-Galactosyl unit and it also contained a minor 2,3,6-α-d-Galatopyranan units and 4-β-d-Mannopyranan residues.

{Reference Type}: Journal Article
{Author}: Zhang, An-Qiang; Zhang, Yang; Yang, Jun-Hong; Sun, Pei-Long
{Year}: 2013
{Title}: Structural elucidation of a novel heteropolysaccharide from the fruiting bodies of Pleurotus eryngii
{Tag}: 0
{Star}: 0
{Place Published}: Langford Lane, Kidlington, Oxford, OX5 1GB, United Kingdom
{Journal}: Carbohydrate Polymers
{Volume}: 92
{Issue}: 2
{Pages}: 2239-2244
{Date Displayed}: 2013
{ISBN/ISSN}: 01448617
{Original Publication}: Elsevier Ltd
{Keywords}: Nuclear magnetic resonance spectroscopy; Alkylation; High performance liquid chromatography; Size exclusion chromatography
{Abstract}: A novel water-soluble heteropolysaccharide (PEPS1), with a molecular weight of 1.88   104 Da as determined by high performance liquid chromatography (HPLC), specifically size exclusion chromatography, was isolated from the fruiting bodies of Pleurotus eryngii by hot water extraction and further purification by DEAE Sepharose Fast Flow and Sephacryl S-300 and S-100 High-Resolution chromatography. By use of compositional analysis, methylation analysis, together with 1H, 13C NMR and 2D NMR spectroscopy including COSY, TOCSY, HMQC, HMBC and NOESY experiments, the PEPS1 was identified as a heteropolysaccharide that consists of a -d-(1   6)-Galactopyranan backbone with a -d-Mannosyl unit on O-2 of the 2,6-di-O-substituted-d-Galactosyl units, -(1   6)-3-O-Me-d- Galactopyranan backbone with a terminal -3-O-Me-d-Galactosyl unit and it also contained a minor 2,3,6--d-Galatopyranan units and 4--d-Mannopyranan residues.   2012 Elsevier Ltd.
{Notes}: Compilation and indexing terms, Copyright 2013 Elsevier Inc.
20130716028631
2D-NMR spectroscopy
Compositional analysis
Fruiting bodies
Heteropolysaccharide
High-resolution chromatographies
Hot water extraction
Methylation analysis
NMR analysis
Pleurotus eryngii
Structural elucidation
{Author Address}: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China