{Reference Type}: Journal Article
{Author}: Zhang, An-Qiang; Xu, Mei; Fu, Li; Sun, Pei-Long
{Year}: 2013
{Title}: Structural elucidation of a novel mannogalactan isolated from the fruiting bodies of Pleurotus geesteranus
{Tag}: 0
{Star}: 0
{Place Published}: Langford Lane, Kidlington, Oxford, OX5 1GB, United Kingdom
{Journal}: Carbohydrate Polymers
{Volume}: 92
{Issue}: 1
{Pages}: 236-240
{Date Displayed}: 2013
{ISBN/ISSN}: 01448617
{Original Publication}: Elsevier Ltd
{Keywords}: Structural analysis; Alkylation; High performance liquid chromatography; Nuclear magnetic resonance spectroscopy; Polysaccharides
{Abstract}: A novel water-soluble polysaccharide named PGPB-1, with a molecular weight of 1.3   104 Da as determined by high-performance liquid chromatography (HPLC), was isolated from the fruiting bodies of Pleurotus geesteranus by boiling water as well as DEAE-Sepharose Fast Flow and High-Resolution Sephacryl S-300. On the bases of compositional analysis, methylation analysis and NMR spectroscopy (1H NMR, 13C NMR, 2D-COSY, TOCSY, HMQC, HMBC and NOESY spectra) analysis, the structural elucidation of PGPB-1 consisted of a -d-(1   6)-galactopyranan and a -d-3-O-Me-d-galactosyl unit backbone with a -d-Mannosyl unit on O-2 of the 2,6-di-O-substituted-d-galactosyl units. The repeating unit of the polysaccharide was established as: PGPB-1 also contains a minor proportion of 6-linked-d-Glcp.   2012 Elsevier Ltd. All rights reserved.
{Notes}: Compilation and indexing terms, Copyright 2013 Elsevier Inc.
20125015779567
Boiling water
Compositional analysis
Fruiting bodies
High resolution
Methylation analysis
Pleurotus
Repeating unit
Structural elucidation
Water-soluble polysaccharides
{Author Address}: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China

{Reference Type}: Journal Article
{Author}: He, P.; Geng, L.; Mao, D.; Wang, Z.; Xu, C.
{Year}: 2013
{Title}: Production, preliminary characterization, and bioactivities of exopolysaccharides from Pleurotus geesteranus 5(#)
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=23215658&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Prep Biochem Biotechnol
{Volume}: 43
{Issue}: 1
{Pages}: 108-22
{DOI}: 10.1080/10826068.2012.712078
{Date Displayed}: 2013
{Date}: 2013-01-20
{Type of Work}: Journal Article
{Accession Number}: 23215658
{Abstract}: The optimal culture conditions of exopolysaccharides (EPS) production in submerged culture medium by Pleurotus geesteranus 5(#) were determined using an orthogonal matrix method. The optimal defined medium (per liter) was 60.0 g maltose, 5.0 g tryptone, 1 mM NaCl, 5 mM KH(2)PO(4), and initial pH 6.0 at 28 degrees C. In the optimal culture medium, the maximum EPS production was 16.97 g/L in a shake flask. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography (SEC), and their molecular characteristics were examined by a multiangle laser-light scattering (MALLS) and refractive index (RI) detector system. The approximate weight-average molar masses of the Fr-I and Fr-II of EPS were determined to be 3.263 x 10(4) and 5.738 x 10(3) g/mol, respectively. The low values of polydispersity ratio (1.176 and 1.124 for Fr-I and Fr-II, respectively) of EPSs mean that these EPS molecules exist much less dispersed in aqueous solution without forming large aggregates. Furthermore, the experiments in vitro indicated that P. geesteranus 5(#) EPS exhibit high antitumor and antioxidative effects.
{Author Address}: College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, PR China.
{Language}: eng

{Reference Type}: Journal Article
{Author}: Zhang, A. Q.; Zhang, Y.; Yang, J. H.; Sun, P. L.
{Year}: 2013
{Title}: Structural elucidation of a novel heteropolysaccharide from the fruiting bodies of Pleurotus eryngii
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=23399283&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Carbohydr Polym
{Volume}: 92
{Issue}: 2
{Pages}: 2239-44
{DOI}: 10.1016/j.carbpol.2012.11.069
{Date Displayed}: 2013 Feb 15
{Date}: 2013-02-15
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't
{Original Publication}: Copyright (c) 2012 Elsevier Ltd. All rights reserved.
{Accession Number}: 23399283
{Abstract}: A novel water-soluble heteropolysaccharide (PEPS1), with a molecular weight of 1.88x10(4) Da as determined by high performance liquid chromatography (HPLC), specifically size exclusion chromatography, was isolated from the fruiting bodies of Pleurotus eryngii by hot water extraction and further purification by DEAE Sepharose Fast Flow and Sephacryl S-300 and S-100 High-Resolution chromatography. By use of compositional analysis, methylation analysis, together with (1)H, (13)C NMR and 2D NMR spectroscopy including COSY, TOCSY, HMQC, HMBC and NOESY experiments, the PEPS1 was identified as a heteropolysaccharide that consists of   a alpha-D-(1-->6)-Galactopyranan backbone with a beta-D-Mannosyl unit on O-2 of the 2,6-di-O-substituted-D-Galactosyl units, alpha-(1-->6)-3-O-Me-D-Galactopyranan backbone with a terminal alpha-3-O-Me-D-Galactosyl unit and it also contained a minor 2,3,6-alpha-D-Galatopyranan units and 4-beta-D-Mannopyranan residues.
{Author Address}: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
{Language}: eng

{Reference Type}: Journal Article
{Author}: Song, Dan; He, Zhenyue; Wang, Chenhao; Yuan, Fengjiao; Dong, Ping; Zhang, Weiyun
{Year}: 2013
{Title}: Regulation of the exopolysaccharide from an anamorph of Cordyceps sinensis on dendritic cell sarcoma (DCS) cell line
{Tag}: 0
{Star}: 0
{Journal}: EUROPEAN JOURNAL OF NUTRITION
{Volume}: 52
{Issue}: 2
{Pages}: 687-694
{ISBN/ISSN}: 1436-6207
{Keywords}: NF-KAPPA-B; TUMOR-BEARING MICE; FRACTION EPSF; DIFFERENTIATION; EXPRESSION; CANCER; PHOSPHORYLATION; POLYSACCHARIDES; TRANSDUCERS; IMMUNOCYTES; Exopolysaccharide; Cordyceps sinensis; Dendritic cell; JAK2/STAT3; NF-kappa B
{Abstract}: Cordyceps sinensis has been regarded as a precious tonic food and herbal medicine in China for thousands of years. The exopolysaccharide (EPS) from an anamorph of Cordyceps sinensis was found to have antitumor immunomodulatory activity. Mature dendritic cells play a role in initiating antitumor immunity, so we try to investigate the effects of EPS on the murine dendritic cell line DCS.
Flow cytometry was used to assay the expression levels of cell surface molecules including major histocompatibility complex (MHC)-II, CD40, CD80, and CD86 of DCS cells and their ability to take up antigens. The ability of DCS cells to activate the proliferation of CTLL-2 T cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method. IL-12 and TNF-alpha levels were detected using ELISA. Western blotting was performed to estimate the levels of phosphorylated Janus kinase 2 (p-JAK2), phosphorylated signal transducer and activator of transcription 3 (p-STAT3), nuclear factor-kappa B (NF-kappa B) p65 and p105.
EPS increased the expressions of MHC-II, CD40, CD80, and CD86 of DCS cells and up-regulated their ability to take up antigens. EPS also enhanced their ability to activate the proliferation of CTLL-2 T cells. IL-12 and TNF-alpha secreted from DCS cells were up-regulated after EPS treatment. Furthermore, EPS significantly caused the decline of p-JAK2 and p-STAT3, significantly increased levels of NF-kappa B p65 in the nucleus and decreased levels of NF-kappa B p105 in the cytoplasm.
EPS may induce DCS cells to exhibit mature characteristics, and the mechanism involved is probably related to the inhibition of the JAK2/STAT3 signal pathway and promotion of the NF-kappa B signal pathway.
{Author Address}: Nanjing Univ, Jiangsu Key Lab Mol Med, Sch Med, Nanjing 210093, Jiangsu, Peoples R China; Nanjing Univ, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China; Nanjing Univ, Jiangsu Key Lab Mol Med, Sch Med, Nanjing 210093, Jiangsu, Peoples R China; Nanjing Univ, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China; Nanjing Univ, Jiangsu Key Lab Mol Med, Sch Med, Nanjing 210093, Jiangsu, Peoples R China; Nanjing Univ, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China; Nanjing Univ, Jiangsu Key Lab Mol Med, Sch Med, Nanjing 210093, Jiangsu, Peoples R China; Nanjing Univ, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China; Nanjing Univ, Jiangsu Key Lab Mol Med, Sch Med, Nanjing 210093, Jiangsu, Peoples R China; Nanjing Univ, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China; Nanjing Univ, Jiangsu Key Lab Mol Med, Sch Med, Nanjing 210093, Jiangsu, Peoples R China; Nanjing Univ, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China