{Reference Type}: Journal Article
{Author}: Musieba, Fredrick; Okoth, Sheila; Mibey, Richard K.; Wanjiku, Stella; Moraa, Knight
{Year}: 2012
{Title}: Suitability of Locally Available Substrates for Cultivation of the Kenyan Indigenous Golden Oyster Mushroom (Pleurotus citrinopileatus Singer)
{Tag}: 0
{Star}: 0
{Place Published}: 244, 5th Avenue # S-207,, New York, NY 10001, United States
{Journal}: American Journal of Food Technology
{Volume}: 7
{Issue}: 10
{Pages}: 650-655
{Date Displayed}: 2012
{ISBN/ISSN}: 15574571
{Original Publication}: Science Publications
{Keywords}: Substrates; Cultivation; Forestry; Gold; Grain (agricultural product); Plastic containers; Shellfish
{Abstract}: The growth and yield performance of indigenous Pleurotus citrinopileatus on selected locally available substrates were determined as a prelude to its domestication. Seven substrates namely bean straw (Phaseolus uulgaris), sawdust of African mahogany (Khaya anthotheca), rice straw (Oryza satiua), maize cobs (Zea mays), wheat straw (Triticum aestiuum), sugarcane bagasse (Saccharum officinarum) and banana leaves (Musa sp.) were tested for their suitability for production of the inhgenous Pleurotus citrinopileatus. Each treatment had 9 plastic bags each containing 1 kg of fresh weight of substrate, each spawned with 50 g of inhgenous oyster mushroom, Pleurotus citrinopileatus. The treatments were arranged in a completely randomized design. Data was collected on days to pinning, fruiting body yeld (fresh weight) and biolopcal efficiency. Data collected was subjected to analysis of variance using minitab version 14. Mean separation was done using Tukey test and effects declared significant at 5% level. The substrates were significantly different (p0.05) in biological efficiency; yield and days to pinning. The best performance was obtained from the bean straw substrate. Maximum yeld (397.71 g kg-1 wet substrate) and biological efficiency of 148% were obtained from bean straw at spawn rate of 5%. This study recommends bean straw as a new substrate for cultivation of Pleurotus citrinopileatus at spawn rate of 5% under local conditions which is being reported for the first time in Kenya.   2012 Academic Journals Inc.
{Notes}: Compilation and indexing terms, Copyright 2013 Elsevier Inc.
20124315595996
Indigenous
Kenya
Oyster mushroom
Pleurotus
Yield
{Author Address}: Kenya Industrial Research and Development Institute, Nairobi, Kenya

{Reference Type}: Journal Article
{Author}: Onyango, B. O.; Palapala, V. A.; Arama, P. F.; Wagai, S. O.; Gichimu, B. M.
{Year}: 2011
{Title}: Suitability of selected supplemented substrates for cultivation of kenyan native wood ear mushrooms (Auricularia auricula)
{Tag}: 0
{Star}: 0
{Place Published}: 244, 5th Avenue # S-207,, New York, NY 10001, United States
{Journal}: American Journal of Food Technology
{Volume}: 6
{Issue}: 5
{Pages}: 395-403
{Date Displayed}: 2011
{ISBN/ISSN}: 15574571
{Original Publication}: Science Publications
{Keywords}: Substrates; Fruits; Plastic containers; Regression analysis; Strain; Wood
{Abstract}: Different organic substrates namely maize cobs, wheat straw, grass straw and sugarcane bagasse supplemented with either wheat or rice bran were evaluated for production of two Kenyan native strains of wood ear mushroom [Auricularia auricula (L. ex Hook.) Underw.]. The objective was to evaluate the suitability of these substrates for cultivation of Kenyan native wood ear mushroom. Plastic bag technology was used with treatments arranged in a completely randomized design replicated three times. Samples of black and brown strains of the wood ear mushroom collected from woody stems of dead and dying trees within Kakamega forest were used in this study. Data was collected on days to pinning, fruit body quality, fruit body yields (number and fresh weight) and biological efficiency. The data collected was subjected to analysis of variance using SAS version 9.1. Mean separation was done using LSD and effects declared significant at 5% level. The two mushroom strains were not significantly (p0.05) different in performance except for the number of fruit bodies where the black strain yielded significantly (p0.05) higher than the brown one. The best performance was obtained from maize cobs and wheat straw substrates supplemented with wheat bran and these combinations were recommended to wood ear mushroom growers.   2011 Academic Journals Inc.
{Notes}: Compilation and indexing terms, Copyright 2013 Elsevier Inc.
20111313872499
Auricularia auricula
Biological efficiency
Fruit bodies
Kenya
Pinning
{Author Address}: Department of Botany and Horticulture, Maseno University, Kenya

{Reference Type}: Journal Article
{Author}: Chhaya, Urvish; Gupte, Akshaya
{Year}: 2013
{Title}: Effect of different cultivation conditions and inducers on the production of laccase by the litter-dwelling fungal isolate Fusarium incarnatum LD-3 under solid substrate fermentation - Springer
{Tag}: 0
{Star}: 0
{Journal}: Annals of Microbiology
{Volume}: Volume 63
{Issue}: Issue 1
{Pages}: pp 215-223
{DOI}: 10.1007/s13213-012-0464-1
{Date}: 2013-03-01
{Abstract}: The litter-dwelling fungus <em class="a-plus-plus">Fusarium incarnatum</em> LD-3 has been identified as a novel producer of laccase. The present work was oriented towards the optimization of various cultivation conditions for maximizing laccase production under solid substrate fermentation. The process parameters were optimized by the “one factor at a time” approach. Maximum laccsase production was obtained at pH?5.0 and at a temperature of 28?°C with 60?% moisture content using rice bran as a substrate. The laccase production was enhanced in the presence of aromatic inducer, i.e. ortho-dianisidine at a concentration of 0.5?mM. Laccase production was further increased by 52.56?% when the medium was supplemented with 2?% (v/v) alcohol. Among the various amino acids tested as a growth factor and nitrogen source, D-Serine and DL-2 Amino n-butyric acid, DL-Alanine and L-Glycine were found to be the most suitable for laccase production. The highest laccase production (1,352.64 U/g) was achieved under optimized conditions, and was 2.1-fold higher than the unoptimized conditions. Thus, the novel litter-dwelling fungal isolate <em class="a-plus-plus">Fusarium incarnatum</em> LD-3 seems to be an efficient producer of laccase and can be further exploited for biotechnological applications. This is the first report on the optimization of cultivation conditions and inducers for laccase production from <em class="a-plus-plus">Fusarium incarnatum</em> LD-3.
{Subject}: Microbiology;Microbial Genetics and Genomics;Microbial Ecology;Fungus Genetics;Medical Microbiology;Applied Microbiology

{Reference Type}: Journal Article
{Author}: Naraian, Ram; Srivastava, Jatin; Garg, Satyendra K.
{Year}: 2011
{Title}: Influence of dairy spent wash (DSW) on different cultivation phases and yield response of two Pleurotus mushrooms - Springer
{Tag}: 0
{Star}: 0
{Journal}: Annals of Microbiology
{Volume}: Volume 61
{Issue}: Issue 4
{Pages}: pp 853-862
{DOI}: 10.1007/s13213-011-0206-9
{Date}: 2011-12-01
{Abstract}: Two commercial oyster mushroom species, <em class="a-plus-plus">Pleurotus florida</em> PF05 (PF) and <em class="a-plus-plus">Pleurotus sajor-caju</em> PS08 (PS), were cultivated on wheat straw (WS), corn cob (CC) and combination of both (CC?+?WS) as basal substrates. These substrates were deliberately supplemented with 5, 10, 20 and 40% of dairy spent wash (DSW) as additive. The influence of DSW on cultivation phases of both mushrooms’ lifecycle and other parameters such as mycelial growth rate, ergosterol production, mycelial run, pin head formation, yield, biological efficiency (BE) and protein content in fruitbodies was evaluated. Different DSW concentrations significantly (<em class="a-plus-plus">P</em>?&lt;?0.05) exhibited continuous variations over different parameters. In contrast to the response of spent wash on PS, PF was superior in every phase and parameter. The lower concentrations up to a certain level unexpectedly influenced the cultivation phases of both mushrooms. The fastest growth rate (6.6?mm day<sup class="a-plus-plus">?1</sup>) and highest ergosterol (437.00?μg g<sup class="a-plus-plus">?1</sup>) were registered at 20% DSW with the combination of CC?+?WS as basal substrate. The earliest mycelial run and pin head formation by PF were recorded within 17 and 21?days at 10% supplementation of WS substrate. The maximum 2,173.62?g fruit body yield and 108.68% BE were obtained from PF at 10% DSW supplementation to CC?+?WS substrates. In addition, the highest content of protein (32.9?mg g<sup class="a-plus-plus">?1</sup>) was achieved in the set of CC?+?WS with 20% DSW added. The results for each parameter of cultivation in supplemented sets were more enhanced compared to unsupplemented sets. The progression of yield response and biological efficiency was noted as flush-I?&gt;?flush-II?&gt;?flush-III with considerable differences. These results suggest the use of DSW at low levels for increasing fruit body yield and BE of <em class="a-plus-plus">Pleurotus</em> mushrooms.
{Subject}: Fungus Genetics;Applied Microbiology;Microbiology;Microbial Genetics and Genomics;Microbial Ecology;Medical Microbiology