{Reference Type}: Journal Article
{Author}: Xie, Chunyan; Liu, Gaixia; Gu, Zhenxin; Fan, Gongjian; Zhang, Lei; Gu, Yingjuan
{Year}: 2009
{Title}: Effects of culture conditions on mycelium biomass and intracellular cordycepin production of Cordyceps militaris in natural medium
{Tag}: 0
{Star}: 0
{Journal}: ANNALS OF MICROBIOLOGY
{Volume}: 59
{Issue}: 2
{Pages}: 293-299
{ISBN/ISSN}: 1590-4261
{Keywords}: SUBMERGED CULTURE; OPTIMIZATION; EXOPOLYSACCHARIDES; CULTIVATION; GROWTH; CELLS; Cordyceps militaris; dry mycelia weight; intracellular cordycepin; culture conditions; Box-Behnken design
{Abstract}: Effects of culture conditions on the growth of mycelium and the production of intracellular cordycepin of Cordyceps militaris were investigated in this study. The natural medium which is a mixture of 53% brown rice paste, 6% beerwort and 42% soybean meal juice was used for the fermentation in shaking flasks. Fermentation temperature, pH and medium capacity, which had been proved to be significant factors, were optimized by Box-Behnken design. Results showed that dry mycelia weight (DMW) and cordycepin yield (CY) was varied with the transformation of culture conditions. The highest DMW (19.1 g/L) and CY (1.8 mg/g) would be obtained at the condition of fermentation temperature 28 degrees C, pH 6.2 and medium capacity 57 mL.
{Author Address}: Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China; Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China; Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China; Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China; Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China; Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China

{Reference Type}: Journal Article
{Year}: 2012
{Title}: [Submerged cultivation and chemical composition of Hericium erinaceus mycelium]
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=23350189&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Antibiot Khimioter
{Volume}: 57
{Issue}: 7-8
{Pages}: 7-11
{Date Displayed}: 2012
{Date}: 2012-01-20
{Type of Work}: English Abstract; Journal Article
{Accession Number}: 23350189
{Abstract}: Submerged cultivation of Hericium erinaceus in various media was studied. The yield of the biomass was shown to depend mainly on the carbon source, whereas the content of water soluble polysaccharides depended mainly on the nitrogen source.   The optimal medium composition provided the biomass yield of 21-23 g/l in 7 days. The biomass was characterized by the content of total protein, lipids and carbohydrates. In addition, the amino acid composition of the biomass was determined and shown to meet all the requirements of FAO/WHO concerning the amounts of essential amino acids (with exception of tryptophane). Oleinic and linoleic acids were identified as the main components of the fatty acids. Two water soluble polysaccharide fractions differing in solubility in aqueous ethanol were isolated and shown to contain rhamnose, fucose, xylose, glucose and galactose in different proportions. Vitamins B1, B2, B6, PP and E, ergosterol and coenzyme Q were also detected in the biomass of H. erinaceus.
{Language}: rus

{Reference Type}: Journal Article
{Year}: 2012
{Title}: [Submerged cultivation of Lentinus edodes strain with broad spectrum biological activity]
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=23477214&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Antibiot Khimioter
{Volume}: 57
{Issue}: 9-10
{Pages}: 3-7
{Date Displayed}: 2012
{Date}: 2012-01-20
{Type of Work}: English Abstract; Journal Article
{Accession Number}: 23477214
{Abstract}: A highly potent strain of Lentinus edodes producing lentinomycin B, an erythadenin metabolite showing hypolipidemic activity, and biologically active water soluble endopolysaccharides was isolated. The optimum composition of the medium for the strain submerged cultivation was developed. The medium provided shorter period of the strain cultivation and a 2-fold increase of the biomass yield and production of the endopolysaccharides up to 21 g/l and 4.8 g/l of the culture fluid respectively. The total fraction of the water soluble polysaccharides isolated from the mycelium contained glucose, galactose, mannose, arabinose and xylose as the neutral monosaccharides. Glucose was the main monosaccharide. The procedure of the strain submerged cultivation provided production of several final substances during a single technological cycle.
{Language}: rus

{Reference Type}: Journal Article
{Author}: Libardi, N. Jr; Gern, R. M.; Furlan, S. A.; Schlosser, D.
{Year}: 2012
{Title}: Laccase production by the aquatic ascomycete Phoma sp. UHH 5-1-03 and the white rot basidiomycete Pleurotus ostreatus DSM 1833 during submerged cultivation on banana peels and enzyme applicability for the removal of endocrine-disrupting chemicals
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=22371062&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Appl Biochem Biotechnol
{Volume}: 167
{Issue}: 5
{Pages}: 1144-56
{DOI}: 10.1007/s12010-012-9601-3
{Date Displayed}: 2012 Jul
{Date}: 2012-07-01
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't
{Accession Number}: 22371062
{Keywords}: Ascomycota/growth & development/*metabolism; Biocatalysis; Biodegradation, Environmental; Culture Techniques/*methods; Endocrine Disruptors/*isolation & purification/metabolism; Enzyme Stability; Fermentation; Immersion; Industrial Waste; Laccase/*biosynthesis/chemistry/isolation & purification/metabolism; Musa/*metabolism; Plant Epidermis/*metabolism; Pleurotus/growth & development/*metabolism
{Abstract}: This work aimed to study the production of laccase from Pleurotus ostreatus DSM 1833 and Phoma sp. UHH 5-1-03 using banana peels as alternative carbon source, the subsequent partial purification and characterization of the enzyme, as well the applicability to degrade endocrine disruptors. The laccase stability with pH   and temperature, the optimum pH, the K (m) and V(max) parameters, and the molar mass were determined. Tests were conducted for assessing the ability of degradation of the endocrine disruptors t-nonylphenol, bisphenol A, and 17alpha-ethinylestradiol. Laccase production of 752 and 1,117 U L(-)(1) was obtained for Phoma sp. and P. ostreatus, respectively. Phoma sp. laccase showed higher stability with temperature and pH. The laccase from both species showed higher affinity by syringaldazine. The culture broth with banana peels induced the production of two isoforms of P. ostreatus (58.7 and 21 kDa) and one of Phoma sp. laccase (72 kDa). In the first day of incubation, the concentrations of bisphenol A and 17alpha-ethinylestradiol were reduced to values close to zero and after 3 days the concentration of t-nonylphenol was reduced in 90% by the P. ostreatus laccase, but there was no reduction in its concentration by the Phoma sp. laccase.
{Author Address}: UFZ, Department of Environmental Microbiology, UFZ, Helmholz Centre for Environmental Research, Permoserstrasse 15, 04318 Leipzig, Germany.
{Language}: eng