{Reference Type}: Journal Article
{Author}: Diamantopoulou, Panagiota; Papanikolaou, Seraphim; Katsarou, Eleni; Komaitis, Michael; Aggelis, George; Philippoussis, Antonios
{Year}: 2012
{Title}: Mushroom Polysaccharides and Lipids Synthesized in Liquid Agitated and Static Cultures. Part II: Study of Volvariella volvacea
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{Star}: 0
{Journal}: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
{Volume}: 167
{Issue}: 7
{Pages}: 1890-1906
{ISBN/ISSN}: 0273-2289
{Keywords}: FATTY-ACID-COMPOSITION; PLEUROTUS-SAJOR-CAJU; GANODERMA-LUCIDUM; SUBMERGED CULTURE; EDIBLE MUSHROOMS; MEDICINAL MUSHROOM; MYCELIAL GROWTH; FERMENTATION; TEMPERATURE; CULTIVATION; Volvariella volvacea; Biomass; Cellular lipids; Polysaccharides; Submerged cultures
{Abstract}: Volvariella volvacea strains were studied in relation with their ability to produce biomass, lipids and polysaccharides. Firstly, screening of four strains (AMLR 188, 190, 191 and 192) was performed in agar cultures, where the mycelial growth rate of the strains was measured, and in static liquid cultures, where the production of biomass, the biosynthesis of total cellular lipids and the consumption of glucose were monitored. For all strains, biomass production was significant (13-15 g l(-1)) and total lipid in dry weight (%, w/w) ranged from 3 to 12 %. Afterwards, a detailed kinetic analysis of mycelial biomass, extra- and intra- cellular polysaccharides (EPS, IPS, respectively) as well as lipid production by a V. volvacea selected strain was conducted in submerged static and agitated cultures. Maximum values of 15 g l(-1) biomass, similar to 1.0 g l(-1) EPS and 5.5 g l(-1) IPS were recorded. Agitation did not have severe impact on biomass, EPS and IPS production, but it increased total lipid in dry weight quantities. EPS, IPS and lipid in dry weight values decreased with time. Glucose was the major cellular carbohydrate detected. Total fatty acid analysis of cellular lipids was performed for all V. volvacea strains and linoleic acid (Delta 9,12)C18:2 was predominant. Neutral lipids constituted the major fraction of cellular lipids, but their quantity decreased as fermentation proceeded. Phospholipids were the most saturated lipid fraction.
{Author Address}: Natl Agr Res Fdn NAGREF, Lab Edible Fungi, Inst Technol Agr Prod, Athens 14123, Greece; Agr Univ Athens, Dept Food Sci & Technol, Athens 11855, Greece; Natl Agr Res Fdn NAGREF, Lab Edible Fungi, Inst Technol Agr Prod, Athens 14123, Greece; Agr Univ Athens, Dept Food Sci & Technol, Athens 11855, Greece; Univ Patras, Dept Biol, Microbiol Unit, Div Genet Cell & Dev Biol, Patras 26500, Greece; Natl Agr Res Fdn NAGREF, Lab Edible Fungi, Inst Technol Agr Prod, Athens 14123, Greece
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Greece; Greece; Greece

{Reference Type}: Journal Article
{Author}: Libardi Junior, Nelson; Miranda Gern, Regina Maria; Furlan, Sandra Aparecida; Schlosser, Dietmar
{Year}: 2012
{Title}: Laccase Production by the Aquatic Ascomycete Phoma sp UHH 5-1-03 and the White Rot Basidiomycete Pleurotus ostreatus DSM 1833 During Submerged Cultivation on Banana Peels and Enzyme Applicability for the Removal of Endocrine-Disrupting Chemicals
{Tag}: 0
{Star}: 0
{Journal}: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
{Volume}: 167
{Issue}: 5
{Pages}: 1144-1156
{ISBN/ISSN}: 0273-2289
{Keywords}: LIGNOCELLULOSIC WASTES; TRAMETES-VERSICOLOR; ESTROGENIC ACTIVITY; BISPHENOL-A; FUNGI; FERMENTATION; NONYLPHENOL; ISOENZYMES; SUBSTRATE; CULTURES; Laccase; Phoma sp.; Pleurotus ostreatus; Agro-wastes; Endocrine disruptors; Decontamination of waste effluents
{Abstract}: This work aimed to study the production of laccase from Pleurotus ostreatus DSM 1833 and Phoma sp. UHH 5-1-03 using banana peels as alternative carbon source, the subsequent partial purification and characterization of the enzyme, as well the applicability to degrade endocrine disruptors. The laccase stability with pH and temperature, the optimum pH, the K (m) and V (max) parameters, and the molar mass were determined. Tests were conducted for assessing the ability of degradation of the endocrine disruptors t-nonylphenol, bisphenol A, and 17 alpha-ethinylestradiol. Laccase production of 752 and 1,117 U L-1 was obtained for Phoma sp. and P. ostreatus, respectively. Phoma sp. laccase showed higher stability with temperature and pH. The laccase from both species showed higher affinity by syringaldazine. The culture broth with banana peels induced the production of two isoforms of P. ostreatus (58.7 and 21 kDa) and one of Phoma sp. laccase (72 kDa). In the first day of incubation, the concentrations of bisphenol A and 17 alpha-ethinylestradiol were reduced to values close to zero and after 3 days the concentration of t-nonylphenol was reduced in 90% by the P. ostreatus laccase, but there was no reduction in its concentration by the Phoma sp. laccase.
{Author Address}: Univ Regiao Joinville, Dept Chem Engn, UNIVILLE, BR-89201972 Joinville, Brazil; UFZ, Dept Environm Microbiol, Helmholz Ctr Environm Res, D-04318 Leipzig, Germany; Univ Regiao Joinville, Dept Chem Engn, UNIVILLE, BR-89201972 Joinville, Brazil; Univ Regiao Joinville, Dept Chem Engn, UNIVILLE, BR-89201972 Joinville, Brazil; UFZ, Dept Environm Microbiol, Helmholz Ctr Environm Res, D-04318 Leipzig, Germany
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Brazil; Germany

{Reference Type}: Journal Article
{Author}: Qi-he, Chen; Kruegener, Sven; Hirth, Thomas; Rupp, Steffen; Zibek, Susanne
{Year}: 2011
{Title}: Co-cultured Production of Lignin-Modifying Enzymes with White-Rot Fungi
{Tag}: 0
{Star}: 0
{Journal}: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
{Volume}: 165
{Issue}: 2
{Pages}: 700-718
{ISBN/ISSN}: 0273-2289
{Keywords}: SOLID-STATE FERMENTATION; PHANEROCHAETE-CHRYSOSPORIUM; MANGANESE PEROXIDASE; PLEUROTUS-OSTREATUS; WHEAT-STRAW; VERSATILE PEROXIDASE; BJERKANDERA-ADUSTA; DEGRADING ENZYMES; LACCASE ACTIVITY; DEGRADATION; LMEs; White-rot fungi; Co-cultivation; Interspecies interaction; Nutrients regulation
{Abstract}: Co-cultivation was a potential strategy in lignocellulolytic biodegradation with producing high activity enzymes due to their synergistic action. The objective of this study was to investigate the rarely understood effects of co-culturing of two white-rot fungi on lignin-modifying enzymes (LMEs) production. Six species, Bjerkandera adusta, Phlebia radiata, Pleurotus ostreatus, Dichomitus squalens, Hypoxylon fragiforme and Pleurotus eryngii, were cultured in pairs to study the production of LMEs. The paired hyphal interaction observed showed that P. eryngii is not suitable for co-growth. The use of agar plates containing dye RBBR showed elevated decolourisation at the confrontation zone between mycelia. Laccase was significantly stimulated only in the co-culture of P. radiata with D. squalens under submerged cultivation; the highest value was measured after 4 days of incubation (120 U mg(-1)). The improved productions of MnP and LiP were simultaneously observed at the co-culture of P. ostreatus and P. radiata (MnP = 800 nkat L(-1) after 4 days of incubation; LiP = 60 nkat L(-1) after 7 days of incubation), though it was not a good producer of laccase. P. ostreatus appeared to possess specific potential to be used in co-cultured production of LMEs. The phenotype of LMEs production was not only dependent on the species used but also regulated by different nutritions available in the culture medium. The present data will provide evidence for illustrating the regulatory roles of C/N on LMEs production under the co-cultures' circumstances.
{Author Address}: Zhejiang Univ, Dept Food Sci & Nutr, Hangzhou 310029, Zhejiang, Peoples R China; Fraunhofer Inst Interfacial Engn & Biotechnol, D-70569 Stuttgart, Germany; Fraunhofer Inst Interfacial Engn & Biotechnol, D-70569 Stuttgart, Germany; Univ Stuttgart, Inst Interfacial Engn, Stuttgart, Germany; Fraunhofer Inst Interfacial Engn & Biotechnol, D-70569 Stuttgart, Germany; Fraunhofer Inst Interfacial Engn & Biotechnol, D-70569 Stuttgart, Germany
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Germany; Peoples R China; Germany

{Reference Type}: Journal Article
{Author}: Dwivedi, Pallavi; Vivekanand, V.; Pareek, Nidhi; Sharma, Amit; Singh, Rajesh P.
{Year}: 2010
{Title}: Bleach Enhancement of Mixed Wood Pulp by Xylanase-Laccase Concoction Derived Through Co-culture Strategy
{Tag}: 0
{Star}: 0
{Journal}: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
{Volume}: 160
{Issue}: 1
{Pages}: 255-268
{ISBN/ISSN}: 0273-2289
{Keywords}: KRAFT PULP; ENZYME PREPARATIONS; FIBER MORPHOLOGY; EUCALYPTUS PULP; WHEAT-STRAW; LIGNIN; DELIGNIFICATION; DEGRADATION; PURIFICATION; PRETREATMENT; Biobleaching; Penicillium oxalicum; Co-cultivation; Xylanase; Laccase; Pleurotus ostreatus
{Abstract}: Mixed enzyme preparation having both xylanase and laccase activity was evaluated for its bleach enhancing ability of mixed wood pulp. The enzyme was produced through co-cultivation of mutant Penicillium oxalicum SAU(E)-3.510 and Pleurotus ostreatus MTCC 1804 under solid-state fermentation. Bleaching of pulp with mixed enzyme had resulted into a notable decrease in kappa number and increased brightness as compared to xylanase alone. Analysis of bleaching conditions had denoted that 8 IU g(-1) of mixed enzyme preparation (xylanase/laccase, 22: 1) had led into maximal removal of lignin from pulp when bleaching was performed at 10% pulp consistency (55 degrees C, pH 9.0) for 3 h. An overall improvement of 21%, 8%, 3%, and 5% respectively in kappa number, brightness, yellowness, and viscosity of pulp was achieved under derived bleaching conditions. Process of enzymatic bleaching was further ascertained by analyzing the changes occurring in polysaccharide and lignin by HPLC and FTIR. The UV absorption spectrum of the compounds released during enzymatic treatment had denoted a characteristic peak at 280 nm, indicating the presence of lignin in released coloring matter. The changes in fiber morphology following enzymatic delignification were studied by scanning electron microscopy.
{Author Address}: Indian Inst Technol, Dept Biotechnol, Roorkee 247667, Uttar Pradesh, India; Indian Inst Technol, Dept Biotechnol, Roorkee 247667, Uttar Pradesh, India; Indian Inst Technol, Dept Biotechnol, Roorkee 247667, Uttar Pradesh, India; Indian Inst Technol, Dept Biotechnol, Roorkee 247667, Uttar Pradesh, India; Indian Inst Technol, Dept Biotechnol, Roorkee 247667, Uttar Pradesh, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India