{Reference Type}: Journal Article
{Author}: Hui, Tao Wen; Hong, Liu; Gang, Li; Qing, Zhu You; Rui, Qin
{Year}: 2011
{Title}: Apoptosis induced by GanoPoly in human gastric cancer cell line SGC-7901 cells
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{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 10
{Issue}: 79
{Pages}: 18279-18284
{ISBN/ISSN}: 1684-5315
{Keywords}: GANODERMA-LUCIDUM POLYSACCHARIDES; CORDYCEPS-SINENSIS; ANTIOXIDATION ACTIVITY; ANTITUMOR-ACTIVITY; ACTIVATION; GROWTH; INDUCTION; EXTRACT; PATHWAY; MYCELIA; Apoptosis; polysaccharide; human gastric cancer cells
{Abstract}: In order to investigate polysaccharide effect on the cultured human gastric cancer cells (SGC7901), DNA ladder, flow cytometry and western blot were used to examine the morpholog, proliferation and apoptosis of human gastric cancer SGC-7901 cells when they were affected by polysaccharide. Results show that multiplication of the cultured cells was restrained by treating with polysaccharide, especially with long time and higher polysaccharide concentration. The cultured cells performed typical apoptosis both in morphological characters and DNA fragmentation after been treated in 48 h with 10 mg/ml polysaccharide. There were about 40% of the cultured cells present in apoptosis, but the expression of BCL-2 protein was down-regulated, while the concentration of BAX protein was up-regulated. This research indicated that polysaccharide can induce human gastric cancer cells apoptosis and restrain the cells multiplication, which can be used for human gastric cancer's active therapy.
{Author Address}: Wuhan Univ, Dept Gastroenterol, ZhongNan Hosp, Wuhan, Peoples R China; S Cent Univ Nationalities, Engn Res Ctr, Protect & Utilizat Bioresource Ethn Area So China, Coll Life Sci, Wuhan 430074, Peoples R China; S Cent Univ Nationalities, Engn Res Ctr, Protect & Utilizat Bioresource Ethn Area So China, Coll Life Sci, Wuhan 430074, Peoples R China; Wuhan Univ, Dept Gastroenterol, ZhongNan Hosp, Wuhan, Peoples R China; S Cent Univ Nationalities, Engn Res Ctr, Protect & Utilizat Bioresource Ethn Area So China, Coll Life Sci, Wuhan 430074, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Yu, Shi-jun; Zhang, Ying; Li, Chun-ru; Zhang, Qian; Ma, Zhong-you; Fan, Mei-zhen
{Year}: 2011
{Title}: Optimization of ultrasonic extraction of mycelial polysaccharides from Paecilomyces hepiali using response surface methodology and its antioxidant activity
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{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 10
{Issue}: 75
{Pages}: 17241-17250
{ISBN/ISSN}: 1684-5315
{Keywords}: TRADITIONAL CHINESE MEDICINE; ASSISTED EXTRACTION; CRUDE POLYSACCHARIDES; CORDYCEPS-SINENSIS; SUBMERGED CULTURE; PLANT MATERIALS; OFFICINALIS-L.; PURIFICATION; SUBSTANCES; ANTITUMOR; Ultrasonic extraction; polysaccharides; Paecilomyces hepiali; response surface methodology; antioxidant activity
{Abstract}: Ultrasonic technology was applied to extract mycelial polysaccharides from Paecilomyces hepiali and the process was optimized by response surface methodology. Antioxidant activity of polysaccharides was also investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (%DPPHsc). Three independent variables were ratio of water to raw material (x(1)), ultrasonic power (x(2)) and extraction time (x(3)). Statistical analysis indicated that x(1), x(2), x(1)(2), x(3)(2), x(1)x(2), x(1)x(3) and x(2)x(3) had significant effect on the yields. Besides, x(2), x(1)(2) and x(2)(2) shaped the %DPPHsc of polysaccharides significantly. Three dimensional surface plots and contour plots were drawn from the mathematical model. The optimal conditions for yield and %DPPHsc were as follows: condition (1) x(1) = 125 mL/g, x(2) = 300 W, x(3) = 12 min, and condition (2) x(1) = 125 mL/g and x(2) = 500 W, x(3) = 11 min, respectively. Under these conditions, the yield was 9.37%, and %DPPHsc was 45.34%. There existed good agreement between experimental and predicted values.
{Author Address}: Anhui Agr Univ, Anhui Prov Key Lab Microbial Control, Hefei 230036, Anhui, Peoples R China; Anhui Agr Univ, Anhui Prov Key Lab Microbial Control, Hefei 230036, Anhui, Peoples R China; Anhui Agr Univ, Anhui Prov Key Lab Microbial Control, Hefei 230036, Anhui, Peoples R China; Anhui Agr Univ, Anhui Prov Key Lab Microbial Control, Hefei 230036, Anhui, Peoples R China; Anhui Agr Univ, Anhui Prov Key Lab Microbial Control, Hefei 230036, Anhui, Peoples R China; Anhui Univ Sci & Technol, Fengyang 233100, Anhui, Peoples R China; Anhui Agr Univ, Anhui Prov Key Lab Microbial Control, Hefei 230036, Anhui, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Li, Rong; Jiang, Xiao-lu; Guan, Hua-shi
{Year}: 2010
{Title}: Optimization of mycelium biomass and exopolysaccharides production by Hirsutella sp in submerged fermentation and evaluation of exopolysaccharides antibacterial activity
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{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 9
{Issue}: 2
{Pages}: 196-203
{ISBN/ISSN}: 1684-5315
{Keywords}: EXO-BIOPOLYMER PRODUCTION; SOYBEAN CYST-NEMATODE; CORDYCEPS-MILITARIS; CULTURE-CONDITIONS; CHEMICAL-PROPERTIES; SINENSIS; GROWTH; FUNGI; POLYSACCHARIDES; RHOSSILIENSIS; Hirsutella sp.; exopolysaccharides; mycelium biomass; antibacterial activity
{Abstract}: Hirsutella is a hyphomycete that has been used as biopesticide. Many compounds with antimycobacterial activity have been reported. However, there were fewer reports about fermentation condition for the Hirsutella and activity of its exopolysaccharides. In the present investigation, the fermentation condition and medium for mycelia growth and exopolysaccharides production were optimized and the antibacterial activity of exopolysaccharides (EPS) from Hirsutella sp. was studied. The optimized conditions and medium were as follows: initial pH 5.5, potato extract 20% (w/v), sucrose 2.5%, peptone 0.5%, K(2)HPO(4) 0.2%, MgSO(4) 0.05% and fermentation for 4 days. The highest production of EPS and mycelium biomass yield reached 2.17 and 10.06 g/l, respectively. The content of sugar and protein of EPS were 92.7 and 5.2%, respectively. The monosaccharide component of EPS was mannose, galactose and glucose with a molar ratio of 4.0:8.2:1.0. Its molecular weight was 23 kDa. The antibacterial effect of EPS was observed that they were most effective against gram-positive bacterium, especially Bacillus subtilis and Micrococcus tetragenus. The MIC of exopolysaccharides to B. subtilis and M. tetragenus was 1 and 2.5 mg/ml, respectively. These results are helpful to expand the application of Hirsutella sp. as a biological pesticide.
{Author Address}: Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China; Ocean Univ China, Sch Med & Pharmaceut, Qingdao 266003, Peoples R China; Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China; Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Zhou, Yuan; Hong-bo, Shao; Chen, De-yu
{Year}: 2009
{Title}: Effects of organic nitrogen and carbon sources on mycelial growth and polysaccharides production and their optimization in the submerged culture of Grifola umbellate, a Chinese medicinal herb
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{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 8
{Issue}: 20
{Pages}: 5208-5214
{ISBN/ISSN}: 1684-5315
{Keywords}: Grifola umbellate; Chinese medicinal herb. polysaccharides; submerged culture; organic nitrogen source; carbon source; orthogonal design
{Abstract}: Grifola umbellate is a famous and expensive Chinese herb medicine and the main medicinal component is polysaccharide mainly produced by its mycelia. Effects of organic nitrogen and carbon resources on mycelial growth and polysaccharides production of a medicinal mushroom, G. umbellate were studied in the submerged culture system. Soybean meal was selected as the optimal organic nitrogen source for its significant promotion effects on mycelial growth and polysaccharides synthesis based on results of an orthogonal matrix experiment design. Glucose was proved to be a preferred carbon source by comparison with fructose and sucrose. The optimal dosages of soybean meal 5.63% (w/v) and glucose 4.33% (w/v) were obtained, respectively through the curvilinear regressions experiment. The productions of mycelial dry weight, exo-polysaccharide (EPS) and inner polysaccharide (IPS), in the optimal medium, were about 3.16, 1.48 and 2.78 folds higher than those in the basal medium, respectively and culture duration was shortened to half through optimizing the organic nitrogen and carbon sources. These results will be significant contribution to process biochemistry of medicinal fungus in the submerged culture system.
{Author Address}: NW A&F Univ, Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China; NW A&F Univ, Minist Water Resources, Yangling 712100, Peoples R China; NW A&F Univ, Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China; NW A&F Univ, Minist Water Resources, Yangling 712100, Peoples R China; Binzhou Univ, Shandong Prov Key Lab Ecoenvironm Sci Yellow Rive, Binzhou 256603, Peoples R China; Chinese Acad Sci, Yantai Inst Costal Zone Res, Yantai 264003, Peoples R China; Qingdao Univ Sci & Technol, Inst Life Sci, Qingdao 266042, Peoples R China; NW A&F Univ, Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China; NW A&F Univ, Minist Water Resources, Yangling 712100, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China; Peoples R China; Peoples R China; Peoples R China