{Reference Type}: Journal Article
{Author}: Patra, Sukesh; Patra, Pradip; Maity, Kankan K.; Mandal, Soumitra; Bhunia, Sanjay K.; Dey, Biswajit; Devi, K. Sanjana P.; Khatua, Somanjana; Acharya, Krishnendu; Maiti, Tapas K.; Islam, Syed S.
{Year}: 2013
{Title}: A heteroglycan from the mycelia of Pleurotus ostreatus: Structure determination and study of antioxidant properties
{Tag}: 0
{Star}: 0
{Place Published}: Langford Lane, Kidlington, Oxford, OX5 1GB, United Kingdom
{Journal}: Carbohydrate Research
{Volume}: 368
{Pages}: 16-21
{Date Displayed}: 2013
{ISBN/ISSN}: 00086215
{Original Publication}: Elsevier Ltd
{Keywords}: Glucose; Alkylation; Chelation; Free radicals; Oxygen; Structure (composition)
{Abstract}: A heteroglycan (PS) isolated from the mycelia of Pleurotus ostreatus was found to consist of l-fucose, d-mannose, and d-glucose in a molar ratio of nearly 1:2:3. On the basis of acid hydrolysis, methylation analysis, periodate oxidation, and NMR studies (1H, 13C, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, HMBC, and DEPT), the structure of the repeating unit of the PS was determined. The repeating unit had a branched backbone composed of (16)-linked   and   d-glucose and (12)-linked -l-fucose. Branching occurred at C-4 position of (16)-linked -d-glucopyranosyl residue with terminal -d-mannose and C-3 position of (16)-linked -d-glucopyranosyl residue with (16)--d- mannopyranosyl moiety terminated by -d-glucose. The structure of the repeating unit of the PS was proposed as: 6)--D-Glcp-(16)- -D-Glcp-(12)--L-Fucp-(131-D-Glcp- (16)--D-Manp 41-D-Manp. The antioxidant properties of the PS were studied. The scavenging activity of hydroxyl free radicals whose EC50 value of PS at 943 g/mL was determined. Further, EC 50 value of the PS at 53 g/mL was observed for scavenging activity of superoxide free radicals. Chelating effects (54.82%) of ferrous ions were observed at 1 mg/mL of the PS.   2012 Elsevier Ltd. All rights reserved.
{Notes}: Compilation and indexing terms, Copyright 2013 Elsevier Inc.
20130716028413
Antioxidant properties
Heteroglycan
Mycelia
NMR studies
Pleurotus ostreatus
{Author Address}: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India

{Reference Type}: Journal Article
{Author}: Wu, Su-Qian; Gao, Shang-Long; Liu, Hong-Hong; Sun, Xin-Yi; Hao, Long; Jia, Le; Pang, Li-Fei; Jia, Shou-Hua; Jia, Meng-Shi
{Year}: 2013
{Title}: Intracellular polysaccharide and its antioxidant activity by Pleurotus citrinopileatus SM-01
{Tag}: 0
{Star}: 0
{Pages}: 1-9
{Date Displayed}: 2013
{ISBN/ISSN}: 15985032
{Keywords}: Oxygen; Aldehydes; Amino acids
{Abstract}: The extraction parameters of intracellular polysaccharide (IPS) from Pleurotus citrinopileatus SM-01 mycelia were optimized, and the in vitro and in vivo antioxidant activities of IPS were investigated. The optimum conditions of IPS extraction were predicted to be an ultrasonic treatment time of 664.09 s, precipitation time of 23.03 h and pH 7.36, and IPS yield was estimated at 16.13%. The in vitro inhibition effects of IPS at a dosage of 5 g/L on the superoxide anion, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals were 73.964.62%, 69.24.37%, and 50.754.39%, respectively, which were 72.565.08%, 22.831.94%, and 43.933.26% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of IPS was 0.90.07, 69.815.24% higher than that of BHT. The activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and alanine aminotransferase (ALT) in mice blood were 241.3823.19, 454.9542.39, 60.325.16, and 32.392.54 U/mL, respectively, and the malonaldehyde (MDA) level was 9.540.72 nmol/mL. The results provided a reference for the large-scale extraction of IPS by P. citrinopileatus SM-01 in industrial fermentation, suggesting that the IPS can be used as a potential antioxidant, which enhances adaptive immune responses.   2013 The Polymer Society of Korea and Springer Sciene+Business Media Dordrecht.
{Notes}: Compilation and indexing terms, Copyright 2013 Elsevier Inc.
IP52383262
1 ,1-diphenyl-2-picrylhydrazyl
Adaptive immune response
Alanine aminotransferase
Antioxidant activities
Butylated hydroxytoluene
Glutathione peroxidase
Hydroxyl radicals
Industrial fermentation
Inhibition effect
In-vitro
Malonaldehyde
Optimum conditions
Pleurotus
Reducing power
Superoxide anions
Superoxide dismutases
Ultrasonic treatments
{Author Address}: College of Life Science, Shandong Agricultural University, Taian, Shandong, 271018, China

{Reference Type}: Journal Article
{Author}: Patra, S.; Patra, P.; Maity, K. K.; Mandal, S.; Bhunia, S. K.; Dey, B.; Devi, K. S.; Khatua, S.; Acharya, K.; Maiti, T. K.; Islam, S. S.
{Year}: 2013
{Title}: A heteroglycan from the mycelia of Pleurotus ostreatus: structure determination and study of antioxidant properties
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=23314300&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Carbohydr Res
{Volume}: 368
{Pages}: 16-21
{DOI}: 10.1016/j.carres.2012.12.003
{Date Displayed}: 2013 Mar 7
{Date}: 2013-03-07
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't
{Original Publication}: Copyright (c) 2013 Elsevier Ltd. All rights reserved.
{Accession Number}: 23314300
{Abstract}: A heteroglycan (PS) isolated from the mycelia of Pleurotus ostreatus was found to consist of l-fucose, d-mannose, and d-glucose in a molar ratio of nearly 1:2:3. On the basis of acid hydrolysis, methylation analysis, periodate oxidation, and NMR studies (1H, 13C, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, HMBC, and DEPT), the structure of the repeating unit of the PS was determined. The repeating unit had   a branched backbone composed of (1-->6)-linked alpha and betad-glucose and (1-->2)-linked alpha-l-fucose. Branching occurred at C-4 position of (1-->6)-linked alpha-d-glucopyranosyl residue with terminal beta-d-mannose and C-3 position of (1-->6)-linked beta-d-glucopyranosyl residue with (1-->6)-alpha-d-mannopyranosyl moiety terminated by alpha-d-glucose. The structure of the repeating unit of the PS was proposed as: [formula: see text]. The antioxidant properties of the PS were studied. The scavenging activity of hydroxyl free radicals whose EC50 value of PS at 943 mug/mL was determined. Further, EC50 value of the PS at 53 mug/mL was observed for scavenging activity of superoxide free radicals. Chelating effects (54.82%) of ferrous ions were observed at 1mg/mL of the PS.
{Author Address}: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
{Language}: eng