{Reference Type}: Journal Article

{Author}: Zi-Hua Han；Jian-Min Ye；Guan-Fu Wang

{Year}: 2013

{Title}:Evaluation of in vivo antioxidant activity of Hericium erinaceus polysaccharides

{Tag}: 0

{Star}: 0

{Volume}52:

{Issue}: 0

{Pages}: 66-71

{DOL}: http://dx.doi.org/10.1016/j.ijbiomac.2012.09.009

{ISBN/ISSN}:0141-8130

{Keywords}: Antioxidant；HPLC；Hericium erinaceus polysaccharides；Kidney；Ischemia reperfusion；SOD

{Abstract}: Hericium erinaceus polysaccharide (HEP) is a traditional Chinese medicine. In the present study, chemical composition and antioxidant activity of HEP was investigated. HPLC analysis showed that the HEP was composed of xylose (7.8%), ribose (2.7%), glucose (68.4%), arabinose (11.3%), galactose (2.5%) and mannose (5.2%). HEP was pre-administered to mice by gavage at a dose of 300 mg/kg for 15 days. Results found that HEP preadministration resulted in a significant decline in blood urea nitrogen (BUN), serum creatinine (Scr) and increase in creatinine clearance (CrCI) levels in HEP-pretreated group compared to renal ischemia reperfusion (IR) group. Malondialdehyde (MDA) level significantly increased, whereas Level of reduced glutathione (GSH) markedly decreased in renal IR animals. These results indicate that IR induced renal oxidative injury damage, as indicated by a increase in MDA level, and decrease in GSH level as well as the antioxidant enzymes activity. Such effects reflect that HEP can significantly decrease lipid peroxidation level and increase antioxidant enzymes activities in experimental animals.

{Author Address}:   http://www.sciencedirect.com

{Language}: English

{Reference Type}: Journal Article

{Author}: Zhen-Yuan Zhu；Yang Liu；Chuan-Ling Si；Jing Yuan；Qiang Lv；Yuan-Yuan Li；Guo-Ling Dong；An-Jun Liu；Yong-Min Zhang

{Year}: 2013

{Title}:Sulfated modification of the polysaccharide from Cordyceps_gunnii mycelia and its biological activities

{Tag}: 0

{Star}: 0

{Volume}: 92

{Issue}: 1

{Pages}: 872-876

{DOL}: http://dx.doi.org/10.1016/j.carbpol.2012.10.007

{ISBN/ISSN}:0144-8617

{Keywords}: Polysaccharide；Anti-tumor activity；Sulfated modification；Cordyceps_gunnii；Anti-oxidant activity

{Abstract}: A chemically new sulfated polysaccharide (SPS50) was prepared from the water soluble polysaccharide (PS50), isolated from Cordyceps_gunnii mycelia, by concentrated sulfuric acid method. The yield of crude SPS50 was 62.34% and its specific rotation was [α]D20 = −36.75°. The structural characteristics of this chemically sulfated polysaccharide were determined based on the infrared analysis (IR), high performance liquid chromatography (HPLC) and sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE). Its biological properties including anti-oxidant and anti-tumor activities were also investigated. The results showed that the anti-oxidant capacity of SPS50 was not as good as SP50 and the anti-tumor activity of SPS50 was much better than PS50. SPS50 showed evident growth inhibition on K562 cells. The tumor inhibition ratio of SPS50 against K562 cells was 69.92%.

{Author Address}:   http://www.sciencedirect.com

{Language}: English

{Reference Type}: Journal Article

{Author}: Zuofa Zhang；Guoying Lv；Weiqiang He；Liangen Shi；Huijuan Pan；Leifa Fan

{Year}: 2013

{Title}:Effects of extraction methods on the antioxidant activities of polysaccharides obtained from Flammulina velutipes

{Tag}: 0

{Star}: 0

{Volume}: 98

{Issue}: 2

{Pages}: 1524-1531

{DOL}: http://dx.doi.org/10.1016/j.carbpol.2013.07.052

{ISBN/ISSN}:0144-8617

{Keywords}: Polysaccharide；Antioxidant activity；Flammulina velutipes；Extraction method

{Abstract}: Four polysaccharides (CFP, UFP, MFP and EFP) were extracted from Flammulina velutipes using hot water, ultrasonic, microwave or enzymatic methods optimized by orthogonal test. Preliminary structural characterizations were conducted using physicochemical properties. Polysaccharides extracted by all four methods showed similar physicochemical characteristics and FT-IR spectra. However, SEM images of tissues of F. velutipes were significantly different. EFP demonstrated better antioxidant activities against hydroxyl radical as well as improved metal chelating activity. UFP showed higher DPPH scavenging activity, but CFP exhibited higher antioxidant activity in reducing power. Hence, these polysaccharides can be used as natural antioxidants in functional foods or medicine. Further experiments on the biological activities of these four polysaccharides are currently in progress.

{Author Address}:   http://www.sciencedirect.com

{Language}: English