{Reference Type}: Journal Article

{Author}:Dong Wang；Shu-Qing Sun；Wei-Zhen Wu；Shun-Liang Yang；Jian-Ming Tan

{Year}: 2014

{Title}: Characterization of a water-soluble polysaccharide from Boletus edulis and its antitumor and immunomodulatory activities on renal cancer in mice

{Tag}: 0

{Star}: 0

{Volume}:105

{Issue}: 0

{Pages}: 127-134

{ISBN/ISSN}: 0144-8617

{Keywords}: Polysaccharide；Structure；Antitumor；Boletus edulis；Characterization；In vitro immunomodulatory activity

{Abstract}:A polysaccharide (BEP, Mw = 113,432 Da) was purified from Boletus edulis, which had a backbone consisting of (1→6)-linked-α-d-glucopyranosyl, (1→2,6)-linked-α-d-galactopyranosyl, (1→6)-linked-α-d-galactopyranosyl, and (1→3)-linked-α-d-rhamnopyranosyl residues, which were branched at O-2 position of (1→2,6)-linked-α-d-galactopyranosyl residue with a single terminal (1→)-linked-α-l-arabinofuranosyl residue. After 32 days’ BEP administration to Renca tumor bearing mice, the tumor mass of Renca transplanted in mice was significantly repressed. Furthermore, BEP could significantly increase the spleen and thymus indices, stimulate splenocytes proliferation, augment NK cell and CTL activities in spleen, and promote the secretion of the cytokines IL-2 and TNF-α in Renca tumor bearing mice. Meanwhile oral administration of BEP (100 and 400 mg/kg) restored all the altered hematological and biochemical parameters of tumor-bearing mice to normal levels. Thus, these data demonstrate that BEP possesses potential immunomodulatory activity and might be employed as effective therapeutic agents for the prevention of renal caner.

{Database Provider}: Web of Sciencedirect

{Language}: English

{Reference Type}: Journal Article

{Author}: Mitchell E Nuhn；Manfred Binder；Andy F   S Taylor；Roy E Halling；David S Hibbett

{Year}: 2013

{Title}: Phylogenetic overview of the Boletineae

{Tag}: 0

{Star}: 0

{Volume}: 117

{Issue}: 7-8

{Pages}: 479-511

{ISBN/ISSN}: 1878-6146

{Keywords}: Ecology；Systematics；Boletaceae；Paxillaceae；Taxonomic review

{Abstract}:The generic and sub-generic relationships in the Boletineae (Boletales) were studied using nuclear large subunit (nuc-lsu), translation elongation factor 1-alpha (tef1), and DNA directed RNA polymerase largest subunit (RPB1). The Boletineae, with the exclusion of Hydnomerulius pinastri, was strongly supported and the status of the families Boletaceae and Paxillaceae is discussed. Members of the genus Boletus are found throughout the phylogeny, with the majority not closely related to the type species, Boletus edulis. Many of the traditional, morphologically defined genera are not supported as monophyletic and additional sampling and taxonomic revisions are needed. The majority of the Boletineae are confirmed or putatively ectomycorrhizal (ECM), but two putatively mycoparasitic lineages (one lineage of Buchwaldoboletus lignicola and Chalciporus piperatus and the second Pseudoboletus parasiticus) are strongly supported.

{Database Provider}: Web of Sciencedirect

{Language}: English

{Reference Type}: Journal Article

{Author}: Kai-ping Wang；Jun Wang；Qiang Li；Qi-lin Zhang；Ru-xu You；Yao Cheng；Li Luo；Yu Zhang

{Year}: 2014

{Title}: Structural differences and conformational characterization of five bioactive polysaccharides from Lentinus edodes

{Tag}: 0

{Star}: 0

{Volume}: 62

{Issue}: 0

{Pages}: 223-232

{ISBN/ISSN}: 0963-9969

{Keywords}: Polysaccharide；Lentinus edodes；Chemical structure；Chain conformation；Anti-tumor activity

{Abstract}:Five polysaccharide fractions (LST1, LST2, LJT1, LJT2 and LJT3) from Lentinus edodes were isolated and purified. Their molecular weights (Mw) were estimated as 65.5 ~ 697 kDa by using high-performance gel permeation chromatography (HPGPC). The results from monosaccharide analysis indicated that all of them contained glucose as main sugar component. The chemical and spectroscopic analyses implied that these fractions were composed of a main chain consisting of several (2 ~ 6) β-(1 → 3)-d-glucose residues with branching at C6 position. The predicted structural units of the purified polysaccharides were established with different degrees of branching (DB). Congo-red test, X-ray diffraction (XRD) and atomic force microscope (AFM) were used to verify that the natural lentinan contained triple helical spatial conformation. Furthermore, the results from anti-tumor tests in vitro and in vivo revealed that these polysaccharide fractions could have dual anti-tumor effects, including killing tumor cells directly and immunoregulation with low toxicity to the host. Interestingly, the high Mw polysaccharides had smaller DB values and displayed greater anti-tumor effects.

{Database Provider}: Web of Sciencedirect

{Language}: English