{Reference Type}: Journal Article
{Author}: Liang, Jun F.; Yang, Zhu L.
{Year}: 2013
{Title}: Lepiota nigrosquamosa, a new species from China
{Tag}: 0
{Star}: 0
{Journal}: NOVA HEDWIGIA
{Volume}: 96
{Issue}: 1-2
{Pages}: 213-220
{ISBN/ISSN}: 0029-5035
{Keywords}: BAYESIAN PHYLOGENETIC INFERENCE; AGARICACEAE; CRISTATA; Agaricales; Agaricaceae; lepiotaceous fungi; taxonomy
{Abstract}: A new species, Lepiota nigrosquamosa, is described from southwestern China. It is characterized by black brownish to black squamules on its pileus and stipe, fusiform or broadly fusiform spores, and a pileus covering of a trichoderm consisting of elongate, subcylindrical terminal elements in between with short clavate elements.
{Author Address}: Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China; Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Da, Silva AF; Sartori, D.; Macedo, FC Jr; Ribeiro, L. R.; Fungaro, M. H.; Mantovani, M. S.
{Year}: 2013
{Title}: Effects of beta-glucan extracted from Agaricus blazei on the expression of ERCC5, CASP9, and CYP1A1 genes and metabolic profile in HepG2 cells
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=23424205&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Hum Exp Toxicol
{DOI}: 10.1177/0960327112468173
{Date Displayed}: 2013 Feb 19
{Date}: 2013-02-19
{Type of Work}: JOURNAL ARTICLE
{Accession Number}: 23424205
{Abstract}: The polysaccharide beta-glucan has biological properties that stimulate the immune system and can prevent chronic pathologies, including cancer. It has been   shown to prevent damage to DNA caused by the chemical and physical agents to which humans are exposed. However, the mechanism of beta-glucan remains poorly understood. The objective of the present study was to verify the protective effect of beta-glucan on the expression of the genes ERCC5 (involved in excision   repair of DNA damage), CASP9 (involved in apoptosis), and CYP1A1 (involved in the metabolism of xenobiotics) using real-time polymerase chain reaction and perform   metabolic profile measurements on the HepG2 cells. Cells were exposed to only benzo[a]pyrene (B[a]P), beta-glucan, or a combination of B[a]P with beta-glucan.   The results demonstrated that 50 microg/mL beta-glucan significantly repressed the expression of the ERCC5 gene when compared with the untreated control cells in these conditions. No change was found in the CASP9 transcript level. However,   the CYP1A1 gene expression was also induced by HepG2 cells exposed to B[a]P only   or in association with beta-glucan, showing its effective protector against damage caused by B[a]P, while HepG2 cells exposed to only beta-glucan did not show CYP1A1 modulation. The metabolic profiles showed moderate bioenergetic metabolism with an increase in the metabolites involved in bioenergetic metabolism (alanine, glutamate, creatine and phosphocholine) in cells treated with beta-glucan and to a lesser extent treated with B[a]P. Thus, these results demonstrate that the chemopreventive activity of beta-glucan may modulate bioenergetic metabolism and gene expression.
{Author Address}: 1Universidade Estadual de Londrina, Londrina, Brazil.
{Language}: ENG

{Reference Type}: Journal Article
{Author}: Ando, Y.; Nakazawa, T.; Oka, K.; Nakahori, K.; Kamada, T.
{Year}: 2013
{Title}: Cc.snf5, a gene encoding a putative component of the SWI/SNF chromatin remodeling complex, is essential for sexual development in the agaricomycete Coprinopsis cinerea
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=23078835&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Fungal Genet Biol
{Volume}: 50
{Pages}: 82-9
{DOI}: 10.1016/j.fgb.2012.09.010
{Date Displayed}: 2013 Jan
{Date}: 2013-01-01
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't
{Original Publication}: Copyright (c) 2012 Elsevier Inc. All rights reserved.
{Accession Number}: 23078835
{Abstract}: We characterized a Coprinopsis cinerea mutant strain, Spe20, defective in fruiting initiation, which was isolated after restriction enzyme-mediated integration (REMI) mutagenesis of a homokaryotic fruiting strain, 326. A plasmid   rescue followed by complementation experiments, RACE, and cDNA analyses revealed   that the gene, a mutation of which is responsible for the phenotype, is predicted to encode a protein that exhibits a high similarity to yeast Snf5p, a key component of the chromatin remodeling complex SWI/SNF, and named Cc.snf5. Cc.Snf5 is, however, different from Snf5p in that the former has, in addition to an Snf5   domain comprising N-terminal repeat1 (rp1) and C-terminal repeat2 (rp2) subdomains in a middle region, a GATA Zn-finger domain in a C-terminal region. In strain Spe20, plasmid pPHT1 used for REMI is inserted in the ORF encoding rp2. This raised the possibility that in strain Spe20, the disrupted Cc.Snf5 is functionally active albeit incompletely because it retains rp1. Thus, we disrupted the whole SNF5 domain and its downstream peptide and found that the disruption results in inhibition of not only fruiting initiation but also dikaryon development, a prerequisite for fruiting. We also found that specific disruption of the Zn-finger domain results in inhibition of fruiting initiation.   These results indicate that Cc.Snf5 plays an essential role in sexual development of C. cinerea.
{Author Address}: Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
{Language}: eng