{Reference Type}: Journal Article
{Author}: Chen, Wei-Min; Chai, Hong-Mei; Zhou, Hui-Ming; Tian, Guo-Ting; Li, Shu-Hong; Zhao, Yong-Chang
{Year}: 2012
{Title}: Phylogenetic analysis of the Agrocybe aegerita multispecies complex in Southwest China inferred from ITS and mtSSU rDNA sequences and mating tests
{Tag}: 0
{Star}: 1
{Journal}: ANNALS OF MICROBIOLOGY
{Volume}: 62
{Issue}: 4
{Pages}: 1791-1801
{ISBN/ISSN}: 1590-4261
{Keywords}: RIBOSOMAL-RNA V4; SECONDARY STRUCTURE; V9 DOMAINS; EVOLUTION; DNA; PLEUROTUS; MUSHROOMS; FUNGI; V6; Agrocybe aegerita; Mating test; Mitochondrial small-subunit; Phylogeny
{Abstract}: The diversity of Agrocybe aegerita multispecies complex in Yunnan Province, southwest China was investigated. In total, 23 strains collected from ten districts were classified into three groups through mitochondrial small-subunit (mtSSU) rDNA sequence analysis. Phylogeny of internal transcribed spacer (ITS) combined with mating tests demonstrated that the tested strains included at least two species. Agrocybe salicacola, which is difficult to distinguish from other species in the A. aegerita complex, was first systematically analyzed and identified with molecular characterization. YAASM0594 and YAASM0625 share sequences with YAASM1024, and XW01 in group B, defined according to the mtSSU rDNA sequences, was related more closely to group C in the ITS phylogenetic tree, indicating that group B and CS45 are closely related, with both groups possibly even being the same species. We conclude that a high diversity occurs in Agrocybe aegerita complex than reported previously, and that more novel species would possibly be discovered through molecular phylogeny and mating tests.
{Author Address}: Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650221, Yunnan Province, Peoples R China; Yunnan Prov Key Lab Agr Biotechnol, Kunming 650221, Yunnan, Peoples R China; Minist Agr, Key Lab SW Crop Gene Resources & Germplasm Innova, Kunming 650221, Yunnan, Peoples R China; Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650221, Yunnan Province, Peoples R China; Yunnan Prov Key Lab Agr Biotechnol, Kunming 650221, Yunnan, Peoples R China; Minist Agr, Key Lab SW Crop Gene Resources & Germplasm Innova, Kunming 650221, Yunnan, Peoples R China; Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650221, Yunnan Province, Peoples R China; Yunnan Prov Key Lab Agr Biotechnol, Kunming 650221, Yunnan, Peoples R China; Minist Agr, Key Lab SW Crop Gene Resources & Germplasm Innova, Kunming 650221, Yunnan, Peoples R China; Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650221, Yunnan Province, Peoples R China; Yunnan Prov Key Lab Agr Biotechnol, Kunming 650221, Yunnan, Peoples R China; Minist Agr, Key Lab SW Crop Gene Resources & Germplasm Innova, Kunming 650221, Yunnan, Peoples R China; Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650221, Yunnan Province, Peoples R China; Yunnan Prov Key Lab Agr Biotechnol, Kunming 650221, Yunnan, Peoples R China; Minist Agr, Key Lab SW Crop Gene Resources & Germplasm Innova, Kunming 650221, Yunnan, Peoples R China; Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650221, Yunnan Province, Peoples R China; Yunnan Prov Key Lab Agr Biotechnol, Kunming 650221, Yunnan, Peoples R China; Minist Agr, Key Lab SW Crop Gene Resources & Germplasm Innova, Kunming 650221, Yunnan, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Singh, Ajay Veer; Sharma, Abhinay; Johri, Bhavdish N.
{Year}: 2012
{Title}: Phylogenetic profiling of culturable bacteria associated with early phase of mushroom composting assessed by amplified rDNA restriction analysis
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{Star}: 0
{Journal}: ANNALS OF MICROBIOLOGY
{Volume}: 62
{Issue}: 2
{Pages}: 675-682
{ISBN/ISSN}: 1590-4261
{Keywords}: AGARICUS-BISPORUS; IDENTIFICATION; CULTIVATION; FUNGI; Mushroom composting; Agaricus bisporus; ARDRA; 16S rDNA; Phylogenetic profiling
{Abstract}: The edible mushroom Agaricus bisporus is grown commercially on composted manure/straw mixtures. Mushroom composting is a fermentation process in which various groups of microorganisms play important roles at different stages of composting. The present study was conducted to explore the mesophilic bacterial diversity in the early phase of mushroom composting. Morphologically all the isolated bacteria were either Gram-positive rods, cocci or Gram-negative rods. The functional diversity of the bacterial isolates was examined by plate enzyme assays, siderophore production and antagonistic property. Good enzymatic activity for amylase, cellulase, xylanase and protease was reported for different bacterial isolates. Nine bacterial isolates showed siderophore production activity. During antibiosis assay, most of the isolates inhibited growth of and . However, a consortium of selected bacterial isolates produced good amounts of lytic enzymes (amylase, cellulase, xylanase and protease) in solid state fermentation experiments that might help in enhancing the composting process. Amplified 16S-rDNA restriction analysis (ARDRA) of bacterial isolates indicated that four groups of nine bacterial isolates had 100% similarity in all the restriction profiles. However, other isolates exhibited discriminatory relationships with each other. The present study reveals culturable mesophilic bacterial diversity and community succession in the early phase of mushroom composting process as well as emphasizing the application of a bacterial consortium to enhance the composting process.
{Author Address}: Govind Ballabh Pant Univ Agr & Technol, Ranichauri 249199, Uttarakhand, India; Jawaharlal Nehru Univ, Sch Life Sci, New Delhi 110067, India; Barkatullah Univ, Dept Biotechnol, Bhopal 462026, India
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: India; India; India

{Reference Type}: Journal Article
{Author}: Chung, Sang Jun; Kim, Seung; Sapkota, Kumar; Choi, Bong-Suk; Shin, Changho; Kim, Sung-Jun
{Year}: 2011
{Title}: Expression of recombinant human interleukin-32 in Pleurotus eryngii
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{Star}: 0
{Journal}: ANNALS OF MICROBIOLOGY
{Volume}: 61
{Issue}: 2
{Pages}: 331-338
{ISBN/ISSN}: 1590-4261
{Keywords}: AGROBACTERIUM-TUMEFACIENS; FILAMENTOUS FUNGI; SACCHAROMYCES-CEREVISIAE; MEDIATED TRANSFORMATION; T-DNA; CYTOKINE; IL-32; CELLS; Agrobacterium tumefaciens; Interleukin-32; Pleurotus eryngii; Gene transfer; Gene expression
{Abstract}: Interleukin-32 is a novel human cytokine implicated in several inflammatory and autoimmune diseases. Recombinant interleukin -32 can be produced and found to be useful in many fields. Until now, there has been no report on recombinant hIL-32 expressed in basidomycete fungus, Pleurotus eryngii. In this study, we examined whether the popular edible mushroom Pleurotus eryngii could support the expression of novel protein hIL-32. A binary vector pCAMBIA1304 containing the hIL-32 gene was constructed and introduced into Pleurotus eryngii via Agrobacterium tumefaciens-mediated transformation. The expression of hIL-32 was confirmed by PCR, Southern blot and western blot analysis. The recombinant hIL-32 reached a maximum expression level of 1.9 % of total soluble protein in transgenic mycelia. These results suggest that Pleurotus eryngii expression system can be effective for the production of rhIL-32 at an economically relevant level.
{Author Address}: Chosun Univ, Dept Biotechnol, Kwangju 501759, South Korea; Gwangju Univ, Dept Alternat Med, Kwangju 503703, South Korea; Chosun Univ, Dept Biotechnol, Kwangju 501759, South Korea; Tribhuvan Univ, Cent Dept Zool, Kathmandu, Nepal; Chosun Univ, Dept Biotechnol, Kwangju 501759, South Korea; Gwangju Univ, Dept Sport & Leisure Studies, Kwangju 503703, South Korea; Chosun Univ, Dept Biotechnol, Kwangju 501759, South Korea
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: South Korea; South Korea; South Korea; Nepal

{Reference Type}: Journal Article
{Author}: Shridharani, Sachin M.; Magarakis, Michael; Manson, Paul N.; Singh, Navin K.; Basdag, Basak; Rosson, Gedge D.
{Year}: 2010
{Title}: The Emerging Role of Antineoplastic Agents in the Treatment of Keloids and Hypertrophic Scars A Review
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{Star}: 0
{Journal}: ANNALS OF PLASTIC SURGERY
{Volume}: 64
{Issue}: 3
{Pages}: 355-361
{ISBN/ISSN}: 0148-7043
{Keywords}: COLLAGEN GENE-EXPRESSION; MESSENGER-RNA LEVELS; TOPICAL MITOMYCIN-C; GROWTH-FACTOR-BETA; OF-THE-LITERATURE; INTERFERON-GAMMA; INTRALESIONAL 5-FLUOROURACIL; FIBROBLAST COLLAGEN; IN-VITRO; SCLERODERMA FIBROBLASTS; wound healing; keloids; hypertrophic scars; anti-neoplastic; interferon; mitomycin-c; bleomycin; 5-fluorouracil
{Abstract}: The management of keloids and hypertrophic scars continues to challenge health-care providers. Though both forms of pathologic scarring are distinct entities at the macro and microscopic level, their etiologies and treatment are often similar. Potential treatment approaches are progressing, and combinations of treatment options have been proposed in the literature with promising outcomes. The treatment evolution has reached a level where molecular therapeutic modalities are being investigated. Currently, no gold standard treatment exists. Overall success rates and patient satisfaction seem to be slowly climbing, but additional investigational studies must continue to be performed.
Several studies have investigated antineoplastic agents, and there seems to be a marked improvement in rates of recurrence, patient satisfaction, and overall quality of scar when these agents are used. Intralesional injection and/or wound irrigation with interferon-a2b, interferon-g, mitomycin-C, bleomycin, or 5-fluorouracil seems to have a positive effect on the reduction of pathologic scars. There is mounting evidence that these drugs used alone or in combination therapy, have the potential to be an integral part of the treatment paradigm for hypertrophic scars and keloids.
{Author Address}: <AuCollectiveName>Shridharani, Sachin M