{Reference Type}: Journal Article
{Author}: Li-qiong, Guo; Er-xian, Liu; Jie, Wang; Jun-fang, Lin
{Year}: 2009
{Title}: Development of Highly Efficient Transformation System of Yeast-Like Conidia of Tremella fuciformis
{Tag}: 0
{Star}: 0
{Journal}: AGRICULTURAL SCIENCES IN CHINA
{Volume}: 8
{Issue}: 3
{Pages}: 268-275
{ISBN/ISSN}: 1671-2927
{Keywords}: HYGROMYCIN-B RESISTANCE; PLEUROTUS-OSTREATUS; EXPRESSION SYSTEMS; COPRINUS-CINEREUS; LENTINUS-EDODES; GENE; INTEGRATION; PROMOTER; Tremella fuciformis; PEG-mediated protoplast transformation; yeast-like conidia; hph gene; promoter
{Abstract}: Tremella fuciformis is one of higher basidiomycetes. Its basidiospore can reproduce yeast-like conidia, which is also called the blastospore by budding. The yeast-like conidia of T. fuciformis is monokaryotic and easy to culture by submerged fermentation similar to yeast. Thus, it is a good recipient cell for exogenous gene expression. In this study, the expression plasmid pAN7-1 (containing promoter gpd-An derived from Aspergillus nidulans and selectable marker gene hph conferring resistance to hygromycin B) and plasmid pLg-hph (containing promoter gpd-Le derived from Lentinula edodes and selectable marker gene hph) were transformed into the yeast-like conidia of T. fuciformis by PEG-mediated protoplast transformation, respectively. The primary putative transformants were selected by the sandwich screening method with the selective medium containing 50 mu g mL(-1) hygromycin. The putative transformants were obtained from the primary putative transformants transferred on PDSA plates containing 100 mu g mL-1 hygromycin for second round selection. Experimental results showed that the optimal concentration of PEG 4000 for mediating protoplast transformation was 25%. PCR and Southern blotting confirmed that the selectable marker gene hph was integrated effectively into the genome of the yeast-like conidia of T. fuciformis with plasmid pLg-hph transformation. Its transformation efficiency was 110 transformants per jig DNA, and the hph gene was integrated into the genome of some yeast-like conidia with plasmid pAN7-1 transformation. However, its transformation efficiency was only 9 transformants per mu g DNA. The presence of hph gene in the genome of transformants after 5 generations of sub-culturing on PDSB medium was confirmed by PCR, suggesting that the foreign gene hph was stable during subculture.
{Author Address}: S China Agr Univ, Coll Food Sci, Dept Bioengn, Guangzhou 510640, Guangdong, Peoples R China; S China Agr Univ, Inst Biomass Res, Guangzhou 510640, Guangdong, Peoples R China; S China Agr Univ, Coll Food Sci, Dept Bioengn, Guangzhou 510640, Guangdong, Peoples R China; S China Agr Univ, Coll Food Sci, Dept Bioengn, Guangzhou 510640, Guangdong, Peoples R China; S China Agr Univ, Coll Food Sci, Dept Bioengn, Guangzhou 510640, Guangdong, Peoples R China; S China Agr Univ, Inst Biomass Res, Guangzhou 510640, Guangdong, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Ziems, Jesse R.; Hoback, W. Wyatt; Higley, Leon G.; Hunt, Thomas E.; Fernandes, Odair A.; Bastos, Cristina; De Freitas Bueno, Adeney
{Year}: 2008
{Title}: Second generation European corn borer injury and Irish potato physiology, yield, and quality
{Tag}: 0
{Star}: 0
{Place Published}: 677 South Segoe Road, Madison, WI 53711-1086, United States
{Journal}: Agronomy Journal
{Volume}: 100
{Issue}: 3
{Pages}: 720-725
{Date Displayed}: 2008
{ISBN/ISSN}: 00021962
{Original Publication}: American Society of Agronomy
{Keywords}: Crops; Cultivation; Economic analysis; Harvesting; Physiology
{Abstract}: European corn borer (ECB) [Ostrinia nubilalis (Hubner)] (Lepidoptera: Crambidae) is known to infest Irish potato (Solanum tuberosum L.) but only causes economic damage during the first generation in East Coast potato producing areas. However, in Nebraska, second generation ECB infest potato plants during the bulking period and may reduce yield and/or potato quality. Experiments were conducted in 2001, 2002, and 2003 to examine physiological and yield effects of second generation ECB injury to potato in Nebraska. Pike, Atlantic, and three Frito Lay proprietary varieties (FL1867, FL1879, and FL1833) were used. Experimental plots were infested with four ECB egg masses per plant to simulate ECB infestation by second-generation larvae; controls received no egg masses. Photosynthetic rates, tuber weights, tuber size grades, solids, and fry quality were measured. Potato plants with ECB infestation had significantly reduced photosynthetic rates on ECB-infested stems and on uninfested stems on the same plant when larvae were in the fifth instar. When insects were in the fourth instar, photosynthetic rates were reduced only on ECB-infested stems. In 2001, ECB infestation reduced the average mass of large tubers and increased the amount of small tubers in FL1867 and FL1879. In 2002, significant yield reductions were not observed. Across both years, ECB-infested plots produced fewer large (65- to 100-mm diam.) tubers than control plots. Other tuber properties and chip qualities were unaffected. This study indicates that second generation ECB infestation of approximately 30% infested plants results in economic loss for some chipping varieties and affects tuber bulking. In contrast to east coast growers, Midwest potato farmers must be concerned with second generation ECB. Copyright   2008 by the American Society of Agronomy. All rights reserved.
{Notes}: Compilation and indexing terms, Copyright 2013 Elsevier Inc.
20082311305366
Economic damage
European corn borer (ECB)
{Author Address}: CSS Farms, 2016 32 Rd., Minden, NE 68959, United States

{Reference Type}: Journal Article
{Author}: Angelini, P.; Pagiotti, R.; Venanzoni, R.; Granetti, B.
{Year}: 2009
{Title}: Antifungal and allelopathic effects of Asafoetida against Trichoderma harzianum and Pleurotus spp.
{Tag}: 0
{Star}: 0
{Journal}: ALLELOPATHY JOURNAL
{Volume}: 23
{Issue}: 2
{Pages}: 357-367
{ISBN/ISSN}: 0971-4693
{Keywords}: ANTIMICROBIAL ACTIVITY; FERULIC ACID; CARCINOGENESIS; INHIBITION; PROFILES; ENZYMES; FUNGI; Antagonism; GC/MS; green moulds; MIC; MFC; transmission electron microscopy
{Abstract}: Methanol extract (MeOH) of Asafoetida oleogum-resin was assayed for its in-vitro ability to control Trichoderma harzianum. The thirty-two components of MeOH-extracted resin were identified by GC-MS analysis. The antifungal and allelopathic effects of the MeOH extracts concentrations against T. harzianum and Pleurouts spp., were investigated in dual culture experiments on an agar-based medium. MeOH extract showed fungistatic and fungicidal properties against T harzianum strains and Pleurotus spp. at higher concentrations. In dual culture, all strains of T. harzianum were antagonistic to Pleurotus spp. than in control. When MeOH extracts concentrations was added to the substrate culture, the antagonistic activity of T harzianum against the Pleurotus spp. was moderate (0.625 mu g/ml of MeOH extract) or weak (1.25 mu g/ml of MeOH extract) against the Pleurotus spp. that either completely or partially replaced T harzianum. TEM observations revealed that fungal growth inhibition from the MeOH extract was accompanied by marked morphological and cytological changes.
{Author Address}: Dipartimento Biol Applicata, I-06121 Perugia, Italy; Dipartimento Biol Applicata, I-06121 Perugia, Italy; Dipartimento Biol Applicata, I-06121 Perugia, Italy; Dipartimento Biol Applicata, I-06121 Perugia, Italy
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Italy

{Reference Type}: Journal Article
{Author}: Wang, X. L.; Yang, R. H.; Yao, Y. J.
{Year}: 2011
{Title}: Development of microsatellite markers for Ophiocordyceps sinensis (Ophiocordycipitaceae) using an ISSR-TAIL-PCR method
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=22130274&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Am J Bot
{Volume}: 98
{Issue}: 12
{Pages}: e391-4
{DOI}: 10.3732/ajb.1100176
{Date Displayed}: 2011 Dec
{Date}: 2011-12-01
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't
{Accession Number}: 22130274
{Keywords}: DNA Primers/metabolism; Genetic Loci/genetics; Genetic Markers; Hypocreales/*genetics; Microsatellite Repeats/*genetics; Molecular Sequence Data; Polymerase Chain Reaction/*methods; Polymorphism, Genetic
{Abstract}: PREMISE OF THE STUDY: Microsatellite primers were developed for Ophiocordyceps sinensis, an endangered medicinal fungus endemic to the Tibetan Plateau, to investigate its genetic diversity and population structure. METHODS AND RESULTS:   An inter-simple sequence repeat (ISSR)-thermal asymmetric interlaced (TAIL)-PCR method was established to develop microsatellite markers. A total of 30 perfect and imperfect microsatellites were identified in 48 individuals of O. sinensis from five provinces within China representing different populations. Seventeen loci were polymorphic with two to four alleles per locus, while 13 were monomorphic. CONCLUSIONS: The results indicate that the microsatellite markers developed here may be used in studies of population genetics and conservation biology of O. sinensis. Furthermore, the ISSR-TAIL-PCR method is a simple strategy for microsatellite marker development.
{Author Address}: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China.
{Language}: eng