{Reference Type}: Journal Article
{Author}: Song, H. H.; Chae, H. S.; Oh, S. R.; Lee, H. K.; Chin, Y. W.
{Year}: 2012
{Title}: Anti-inflammatory and anti-allergic effect of Agaricus blazei extract in bone marrow-derived mast cells
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=22928836&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Am J Chin Med
{Volume}: 40
{Issue}: 5
{Pages}: 1073-84
{DOI}: 10.1142/S0192415X12500796
{Date Displayed}: 2012
{Date}: 2012-01-20
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't
{Accession Number}: 22928836
{Keywords}: *Agaricus; Animals; Anti-Allergic Agents/pharmacology/*therapeutic use; Anti-Inflammatory Agents/pharmacology/*therapeutic use; Biological Agents/pharmacology/*therapeutic use; Bone Marrow Cells/drug effects/metabolism; Calcimycin/pharmacology; Cell Degranulation/drug effects; Down-Regulation; Female; Inflammation/*drug therapy/metabolism; Interleukin-6/*biosynthesis; Leukotriene C4/metabolism; Mast Cells/*drug effects/metabolism; Mice; Mice, Inbred BALB C; Phosphorylation; Phytotherapy; Prostaglandin D2/metabolism; Proto-Oncogene Proteins c-akt/metabolism; Tetradecanoylphorbol Acetate/pharmacology; beta-N-Acetylhexosaminidases/metabolism
{Abstract}: In this study, the anti-inflammatory and anti-allergic effects of the chloroform-soluble extract of Agaricus blazei in mouse bone marrow-derived mast cells (BMMCs) were investigated. The chloroform-soluble extract inhibited IL-6 production in PMA plus A23187-stimulated BMMCs, and down-regulated the phosphorylation of Akt. In addition, this extract demonstrated inhibition of the   degranulation of beta-hexosaminidase and the production of IL-6, prostaglandin D(2) and leukotriene C(4) in PMA plus A23187-induced BMMCs. In conclusion, the chloroform-soluble extract of Agaricus blazei exerted anti-inflammatory and anti-allergic activities mediated by influencing IL-6, prostaglandin D(2), leukotriene C(4), and the phosphorylation of Akt.
{Author Address}: Immune Modulator Research Center, Bio-Therapeutics Research Institute, Korea Research Institute of Bioscience and Biotechnology, ChungBuk, Korea.
{Language}: eng

{Reference Type}: Journal Article
{Author}: Zhang, G.; Zeng, X.; Li, C.; Li, J.; Huang, Y.; Han, L.; Wei, J. A.; Huang, H.
{Year}: 2011
{Title}: Inhibition of urinary bladder carcinogenesis by aqueous extract of sclerotia of Polyporus umbellatus fries and polyporus polysaccharide
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21213404&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Am J Chin Med
{Volume}: 39
{Issue}: 1
{Pages}: 135-44
{DOI}: 10.1142/S0192415X11008701
{Date Displayed}: 2011
{Date}: 2011-01-20
{Type of Work}: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
{Accession Number}: 21213404
{Keywords}: Animals; Anticarcinogenic Agents/pharmacology/*therapeutic use; Biological Agents/pharmacology/therapeutic use; Disease Models, Animal; Female; Fungal Structures; Glutathione S-Transferase pi/genetics/*metabolism; NAD(P)H Dehydrogenase (Quinone)/genetics/*metabolism; *Phytotherapy; Polyporus/*chemistry; Polysaccharides/pharmacology/*therapeutic use; RNA, Messenger/metabolism; Rats; Rats, Inbred F344; Up-Regulation; Urinary Bladder Neoplasms/chemically induced/metabolism/*prevention & control
{Abstract}: The study aimed to evaluate inhibition effect of sclerotia of Polyporus umbellatus Fries aqueous extract (SPUE) and polyporus polysaccharide (PPS) on bladder cancer, then to measure their effect on mRNA expression of glutathione S-transferase pi (GSTPi) and NAD(P)H:quinone oxidoreductase 1 (NQO1) in female Fischer-344 rats model. The model rats were induced by N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN) for a period of 8 weeks and saccharin for 12 weeks. SPUE (50 mg/kg, 250 mg/kg, 500 mg/kg) and PPS (28 mg/kg)   were orally administrated to the model rats during the whole study. Compared to the control group, a more preventive effect of SPUE and PPS treatment on bladder   cancer was discovered, higher mRNA upregulation of GSTpi and NQO1 was seen in the treatment group. Furthermore, the GSTPi and NQO1 mRNA upregulated level in the low-dose group (SPUE 50 mg/kg) was at maximum. In brief, SPUE and PPS are highly   effective in inhibiting bladder carcinogenesis in rats, which may be associated with upregulation of GSTPi and NQO1 in the bladder.
{Author Address}: Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, P. R. China.
{Language}: eng

{Reference Type}: Journal Article
{Author}: Niu, Y. C.; Liu, J. C.; Zhao, X. M.; Cao, J.
{Year}: 2009
{Title}: A low molecular weight polysaccharide isolated from Agaricus blazei Murill (LMPAB) exhibits its anti-metastatic effect by down-regulating metalloproteinase-9 and up-regulating Nm23-H1
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19885951&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Am J Chin Med
{Volume}: 37
{Issue}: 5
{Pages}: 909-21
{DOI}: 10.1142/S0192415X09007351
{Date Displayed}: 2009
{Date}: 2009-01-20
{Type of Work}: Journal Article; Research Support, Non-U.S. Gov't
{Accession Number}: 19885951
{Keywords}: Agaricus/*chemistry; Animals; Cell Line, Tumor; Dose-Response Relationship, Drug; Down-Regulation/drug effects; Gene Expression Regulation, Neoplastic/drug effects; Humans; Immunohistochemistry; Lung Neoplasms/*prevention & control/secondary; Male; Matrix Metalloproteinase 9/genetics/*metabolism; Melanoma, Experimental/pathology/prevention & control; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; NM23 Nucleoside Diphosphate Kinases/genetics/*metabolism; Neoplasms, Experimental/pathology/*prevention & control; Polysaccharides/*pharmacology; Reverse Transcriptase Polymerase Chain Reaction; Up-Regulation/drug effects; Xenograft Model Antitumor Assays
{Abstract}: The components of Agaricus blazei Murill (AbM) have been shown to possess antitumor potentials. Herein, we attempted to explore the anti-metastatic effect   and underlying mechanism of a low molecular weight polysaccharide isolated from AbM (LMPAB). Matrigel invasion assay was applied to evaluate the effect of LMPAB   on migration of BEL-7402 hepatic cancer cells in vitro. In vivo, the anti-metastatic effect of LMPAB was investigated in mouse B16 melanoma and a double-grafted SW180 tumor models. mRNA and protein levels of metalloproteinase-9 (MMP-9) or nm23-H1 upon LMPAB treatment were detected by real-time PCR and immunohistochemistry assays. LMPAB significantly reduced the invasion of BEL-7402 cells. In vivo, LMPAB was revealed to decrease lung metastatic foci in mouse B16   melanoma model. In the double-grafted SW180 mouse tumor model, we further demonstrated that intratumoral treatment of LMPAB inhibited the growth of tumor on treated side but also suppresses the regression of metastatic tumors on the non-treated side. Moreover, LMPAB reduced MMP-9 but enhanced nm23-H1 mRNA and protein expression. LMPAB displays anti-metastatic activities, indicating the potential of its clinical application for the prevention and treatment of cancer   metastasis. Its anti-metastatic effect may relate to the modulation on MMP-9 and   nm23-H1.
{Author Address}: Institute of Medicine, Qiqihar Medical College, Qiqihar, China.
{Language}: eng

{Reference Type}: Journal Article
{Author}: Zhou, X.; Luo, L.; Dressel, W.; Shadier, G.; Krumbiegel, D.; Schmidtke, P.; Zepp, F.; Meyer, C. U.
{Year}: 2008
{Title}: Cordycepin is an immunoregulatory active ingredient of Cordyceps sinensis
{URL}: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19051361&query_hl=1
{Tag}: 0
{Star}: 0
{Journal}: Am J Chin Med
{Volume}: 36
{Issue}: 5
{Pages}: 967-80
{DOI}: 10.1142/S0192415X08006387
{Date Displayed}: 2008
{Date}: 2008-01-20
{Type of Work}: Journal Article
{Accession Number}: 19051361
{Keywords}: Adult; Cell Proliferation/drug effects; Cells, Cultured; Cordyceps/chemistry/*immunology; Cytokines/genetics/immunology; Deoxyadenosines/*immunology/pharmacology; Female; Gene Expression/drug effects; Humans; Leukocytes, Mononuclear/drug effects/immunology; Male; Plant Extracts/*immunology/pharmacology; Protein Binding/drug effects; T-Lymphocytes/drug effects/immunology; Transcription Factors/genetics/metabolism
{Abstract}: We have reported that cordycepin, an adenosine derivative from the fungus Cordyceps, increased interleukin (IL)-10 expression, decreased IL-2 expression and suppressed T lymphocyte activity. In the present study, we further characterized the regulatory effects of cordycepin on human immune cells. Moreover, a traditional Chinese drug, Cordyceps sinensis (CS) that contains cordycepin, was also investigated. Cytometric Bead Array (CBA) was used to determine the concentrations of IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, TNF-alpha and IFN-gamma in culture of peripheral blood mononuclear cells (PBMCs). The results showed that both cordycepin and CS up-regulated IL-10, IL-1beta, IL-6, IL-8 and TNF-alpha; at the same time, they suppressed phytohemagglutinin (PHA)-induced production of IL-2, IL-4, IL-5, IFN-gamma and IL-12. As compared to cordycepin, CS displayed its regulatory effects on IL-2 and IL-10 in a similar dose-dependent manner even with higher efficiency. The binding activity of transcription factors in a human monocytic cell line THP-1 was tested by the trans-AM method, and a higher binding activity of SP1 and SP3 was observed in cordycepin or CS treated cells compared to the control. These results led to the opinion that cordycepin and CS pleiotropically affected the actions of immune cells and cytokine network in a similar fashion. Cordycepin could be an important immunoregulatory active ingredient in Cordyceps sinensis. In addition, CS may contain substances which possess synergism with cordycepin, as CS showed a higher efficiency in the production of IL-10 and IL-2 than cordycepin. However, merits of these effects in pharmacology and clinical medicine have yet to be proven and   the precise mechanism of these immune regulatory actions should be researched.
{Author Address}: Laboratory for Paediatric Immunology and Infectious Diseases, Children's Hospital, Johannes Gutenberg University of Mainz, Mainz, Germany. xzhou@uni-mainz.de
{Language}: eng