{Reference Type}: Journal Article

{Author}: Chengshu Wang；Ming-Guang Feng

{Year}: 2014

{Title}:Advances in fundamental and applied studies in China of fungal biocontrol agents for use against arthropod pests

{Tag}: 0

{Star}: 0

{Volume}: 68

{Issue}: 0

{Pages}: 129-135

{DOL}: http://dx.doi.org/10.1016/j.biocontrol.2013.06.017

{ISBN/ISSN}:1049-9644

{Keywords}: Entomopathogenic fungi；Genomics；Population genetics；Genetic engineering；Mass production；Microbial control

{Abstract}: Entomopathogenic fungi, such as Beauveria bassiana and Metarhizium anisopliae, are environmentally friendly biocontrol agents (BCAs) against various arthropod pests. We provide an overview to the past-decade advances in fungal BCA research and application in China. Since 1960s, fungal BCAs have been mass-produced for application and at present, thousands of tons of their formulations are annually applied to control forest, agricultural, greenhouse and grassland insect pests throughout the country. Apart from technical advances in mass production, formulation and application of fungal BCAs, basic studies on the genomics, molecular biology, genetic engineering and population genetics of fungal entomopathogens have rapidly progressed in the past few years in China. The completed genomic studies of M. anisopliae, Metarhizium acridum, B. bassiana and Cordyceps militaris provide profound insights into crucial gene functions, fungal pathogenesis, host–pathogen interactions and mechanisms involved in fungal sexuality. New knowledge gained from the basic studies has been applied to improve fungal virulence and stress tolerance for developing more efficacious and field-persistent mycoinsecticides by means of microbial biotechnology, such as genetic engineering. To alleviate environmental safety concerns, more efforts are needed to generate new data not only on the effects of engineered BCAs on target and non-target arthropods but also on their potential effects on gene flow and genetic recombination before field release.

{Author Address}:   http://www.sciencedirect.com

{Language}: English

{Reference Type}: Journal Article

{Author}: Atsushi Kurahashi；Masayuki Sato；Kozo Nishibori；Fumihiro Fujimori

{Year}: 2014

{Title}:Heat shock protein 9 mRNA expression increases during fruiting body differentiation in Grifola frondosa and other edible mushrooms

{Tag}: 0

{Star}: 0

{Volume}: 55

{Issue}: 2

{Pages}: 98-102

{DOL}: http://dx.doi.org/10.1016/j.myc.2013.06.001

{ISBN/ISSN}:1340-3540

{Keywords}: Basidiomycota；Gene expression；Maitake mushroom；Small heat shock protein

{Abstract}: We isolated a homolog of Schizosaccharomyces pombe HSP9 from Grifola frondosa and designated it Gf.HSP9. The Gf.HSP9 gene consisted of four exons and three introns and encoded 84 amino acid residues. We have also identified related HSP9 genes from Pleurotus eryngii (Pe.HSP9), Hypsizygus marmoreus (Hm.HSP9), and Lentinula edodes (Le.HSP9). The predicted tertiary structures of these HSP9s were the same. In addition, the expression pattern of their mRNA increased during fruiting body in a differentiation-dependent manner. These results suggested that HSP9 was associated with the fruiting body differentiation in these four edible mushrooms.

{Author Address}:   http://www.sciencedirect.com

{Language}: English

{Reference Type}: Journal Article

{Author}: Annegret Kohler；Emilie Tisserant

{Year}: 2014

{Title}:Chapter Two - Exploring the Transcriptome of Mycorrhizal Interactions

{Tag}: 0

{Star}: 0

{Volume}: 70

{Issue}: 0

{Pages}: 53-78

{DOL}: http://dx.doi.org/10.1016/B978-0-12-397940-7.00002-1

{ISBN/ISSN}:0065-2296

{Keywords}: Ectomycorrhiza；Transcriptome；Mycorrhiza；Arbuscular mycorrhiza；RNA-Seq；Next-generation sequencing；Microarrays；RNA；Plant–microbe interaction

{Abstract}: The transcriptome is defined as the sum of all transcripts in a given cell, tissue or organ and can be assessed by different approaches. The most recent ones, microarrays and RNA sequencing, are compared in this chapter with a focus on plant–microbe interactions. The advantages and the specific problems to monitor both partners at the same time by sequencing are discussed. We show that microdissection in combination with transcriptomics is a powerful tool to study interactions. Further, we present an overview of bioinformatic approaches and available algorithms to process RNA-Seq data. Finally, we summarize the transcriptome analyses from mycorrhizal fungi in the genome era, in particular Laccaria bicolor, Tuber melanosporum, Rhizophagus irregularis and Paxillus involutus.

{Author Address}:   http://www.sciencedirect.com

{Language}: English