{Reference Type}: Journal Article

{Author}: Lin Wu;Arend van Peer;Wenhua Song;Hong Wang;Mingjie Chen;Qi Tan;Chunyan Song;Meiyan Zhang;Dapeng Bao

{Year}: 2013

{Title}:Cloning of the Lentinula edodes B mating-type locus and identification of the genetic structure controlling B mating

{Tag}: 0

{Star}: 0

{Volume}: 531

{Issue}: 2

{Pages}: 270-278

{DOL}: http://dx.doi.org/10.1016/j.gene.2013.08.090

{ISBN/ISSN}:0378-1119

{Keywords}: Lentinula edodes;B mating-type locus;Genetic structure;Pheromone receptor

{Abstract}: During the life cycle of heterothallic tetrapolar Agaricomycetes such as Lentinula edodes (Berk.) Pegler, the mating type system, composed of unlinked A and B loci, plays a vital role in controlling sexual development and resulting formation of the fruit body. L. edodes is produced worldwide for consumption and medicinal purposes, and understanding its sexual development is therefore of great importance. A considerable amount of mating type factors has been indicated over the past decades but few genes have actually been identified, and no complete genetic structures of L. edodes B mating-type loci are available. In this study, we cloned the matB regions from two mating compatible L. edodes strains, 939P26 and 939P42. Four pheromone receptors were identified on each new matB region, together with three and four pheromone precursor genes in the respective strains. Gene polymorphism, phylogenetic analysis and distribution of pheromone receptors and pheromone precursors clearly indicate a bipartite matB locus, each sublocus containing a pheromone receptor and one or two pheromone precursors. Detailed sequence comparisons of genetic structures between the matB regions of strains 939P42, 939P26 and a previously reported strain SUP2 further supported this model and allowed identification of the B mating type subloci borders. Mating studies confirmed the control of B mating by the identified pheromone receptors and pheromones in L. edodes.

{Author Address}:   http://www.sciencedirect.com

{Language}: English

{Reference Type}: Journal Article

{Author}: Lin-zhi Kang;Xian-lu Zeng;Zhi-wei Ye;Jun-fang Lin;Li-qiong Guo

{Year}: 2014

{Title}:Compositional analysis of the fruiting body of transgenic Flammulina velutipes producing resveratrol

{Tag}: 0

{Star}: 0

{Volume}: 164

{Issue}: 0

{Pages}: 211-218

{DOL}: http://dx.doi.org/10.1016/j.foodchem.2014.05.023

{ISBN/ISSN}:0308-8146

{Keywords}: Flammulina velutipes;Fruiting body;Composition analysis;Resveratrol

{Abstract}: Two strains of transgenic Flammulina velutipes TF71 and TF7H with 4cl and rs genes with the capability to produce resveratrol were obtained. In the nutrition assessment and analysis of transgenic strain and original strain (F7), the amino acid composition and proximate compositions of fruiting bodies were determined by amino acid automatic instrument and standard methods of the Association of Official Analytical Chemists (AOAC), while 4-coumaric acid, total flavonoids, and resveratrol were extracted by ethyl acetate and quantified by HPLC. Results indicated that significant differences were observed in proximate composition and amino acid between transgenic and original strains, but these detected components were within the normal ranges reported for F. velutipes. Total flavonoids and 4-coumaric acid contents of transgenic strains are lower than F7. Most important of all, resveratrol has been detected from TF71 and TF7H fruiting body but not found in F7, which was firstly produced by a transgenic mushroom.

{Author Address}:   http://www.sciencedirect.com

{Language}: English

{Reference Type}: Journal Article

{Author}: Polashree Khaund;S R Joshi

{Year}: 2014

{Title}:DNA barcoding of wild edible mushrooms consumed by the ethnic tribes of India

{Tag}: 0

{Star}: 0

{Volume}: 550

{Issue}: 1

{Pages}: 123-130

{DOL}: http://dx.doi.org/10.1016/j.gene.2014.08.027

{ISBN/ISSN}:0378-1119

{Keywords}: Wild edible mushrooms;Tribes;Forests;Traditional markets;Multi-loci molecular characterization;DNA barcoding;Meghalaya;India

{Abstract}: Wild edible mushrooms are consumed by the tribes of Meghalaya in the North-Eastern region of India, as part of their ethnic cuisine because of their favored organoleptic characteristics and traditionally known health benefits. Majority of these mushrooms have not yet been characterized in detail and are slowly shrinking in their natural habitats owing to anthropogenic factors and climate change. In the present study, representative specimens of ten morphologically distinct groups of wild edible mushrooms available in the traditional markets and their respective forest habitats, were subjected to multi-loci molecular characterization using SSU, ITS, RPB1 and RPB2 markers. The species identities inferred for the ten mushroom types using the SSU marker matched their morphological description in the case of four morphological groups only whereas the ITS marker successfully resolved the species identity for nine out of the ten mushroom groups under study. Both the protein coding gene markers RPB1 and RPB2 successfully resolved the species identity for three out of the ten morphologically distinct groups. Finally the most likely identity of the wild edible mushrooms under study has been suggested by matching their unique morphological characteristics with the generated DNA barcoding data. The present molecular characterization reveals the ten widely consumed wild mushroom types of Meghalaya, India to be Gomphus floccosus, Lactarius deliciosus, Lactarius volemus, Cantharellus cibarius, Tricholoma viridiolivaceum, Inocybe aff. sphaerospora, Laccaria vinaceoavellanea, Albatrellus ellisii, Ramaria maculatipes and Clavulina cristata. The final species identity generated by the ITS marker matched more accurately with the morphological characteristics/appearance of the specimens indicating the ITS region as a reliable barcode for identifying wild edible mushrooms.

{Author Address}:   http://www.sciencedirect.com

{Language}: English