{Reference Type}: Journal Article

{Author}: Xiaoli Chen;Guanghong Wu;Zhuolie Huang

{Year}: 2013

{Title}:Structural analysis and antioxidant activities of polysaccharides from cultured Cordyceps militaris

{Tag}: 0

{Star}: 0

{Volume}: 58

{Issue}: 0

{Pages}: 18-22

{DOL}: http://dx.doi.org/10.1016/j.ijbiomac.2013.03.041

{ISBN/ISSN}:0141-8130

{Keywords}: Polysaccharides;Cordyceps militaris;Antioxidant activities;Structure analysis

{Abstract}: The polysaccharides extracted by hot water and precipitated by 50% ethanol from the fruiting bodies of cultured Cordyceps militaris (W-CBP50) were introduced. Antioxidant activities of W-CBP50, W-CBP50 I (obtained from W-CBP50 purified by Sephadex G-100 chromatography) and W-CBP50 II (obtained from W-CBP50I purified by Sephadex G-200 chromatography) were evaluated and structural features of W-CBP50 II were analyzed. The polysaccharide W-CBP50 II was able to scavenge the stable free radical 1,1-diphenyl-2-picrylhyrazyl (DPPH), while W-CBP50 and W-CBP50I could scavenge DPPH, hydroxyl and superoxide radical. Structural characteristics of W-CBP50 II were investigated using GPC, GC–MS, 1H NMR and IR. The results showed that it was mainly composed of α-glucose, α-mannose, α-galactose and α-arabinose with α-type glycosidic linkage and the molecular weight of its main component was 8.97 kD.

{Author Address}:   http://www.sciencedirect.com

{Language}: English

{Reference Type}: Journal Article

{Author}: Yanfang Liu；Yuan Zhao；Yan Yang；Qingjiu Tang；Shuai Zhou；Di Wu；Jingsong Zhang

{Year}: 2013

{Title}:Structural characteristics and hypoglycemic activity of polysaccharides from Coprinus comatus

{Tag}: 0

{Star}: 0

{Volume}: 2

{Issue}: 2

{Pages}: 164 - 169

{DOL}: http://dx.doi.org/10.1016/j.bcdf.2013.10.001

{ISBN/ISSN}:2212-6198

{Keywords}: Purificatio;Coprinus comatus;HPSEC-MALLS;Hypoglycemic activity

{Abstract}: The effects of grinding process on the extraction of polysaccharide from Coprinus comatus were investigated. The extracts named as CCPF and CCPP were obtained from the fragments and the powder of the C. comatus fruiting bodies, respectively. The comparison on the chemical analysis of CCPF and CCPP showed that reducing particle size could dramatically improve the extract yield and content of polysaccharide fractions, which resulted from the increasing of high molecular weight (Mw) fractions. The high Mw fraction CCPP-1 was isolated using the ethanol precipitating method. Its molecular weight was estimated to be 2.44×107 g/mol by high-performance size exclusion chromatography-multi angle laser light scattering (HPSEC-MALLS). CCPP-1 took compact conformation in aqueous solution. Structural analysis showed that CCPP-1 was α-d-(1→4)-glucan with branches at C-6 consisting of non-reducing terminal approximately every fourteen residues. The hypoglycemic activity study revealed that CCPP-1 had no effect on reducing blood sugar. The crude polysaccharide extracts CCPF showed significant hypoglycemic activity.

{Author Address}:   http://www.sciencedirect.com

{Language}: English

{Reference Type}: Journal Article

{Author}: Xiaoli Chen;Guanghong Wu;Zhuolie Huang

{Year}: 2013

{Title}:Structural analysis and antioxidant activities of polysaccharides from cultured Cordyceps militaris

{Tag}: 0

{Star}: 0

{Volume}: 58

{Issue}: 0

{Pages}: 18-22

{DOL}: http://dx.doi.org/10.1016/j.ijbiomac.2013.03.041

{ISBN/ISSN}:0141-8130

{Keywords}: Polysaccharides;Cordyceps militaris;Antioxidant activities;Structure analysis

{Abstract}: The polysaccharides extracted by hot water and precipitated by 50% ethanol from the fruiting bodies of cultured Cordyceps militaris (W-CBP50) were introduced. Antioxidant activities of W-CBP50, W-CBP50 I (obtained from W-CBP50 purified by Sephadex G-100 chromatography) and W-CBP50 II (obtained from W-CBP50I purified by Sephadex G-200 chromatography) were evaluated and structural features of W-CBP50 II were analyzed. The polysaccharide W-CBP50 II was able to scavenge the stable free radical 1,1-diphenyl-2-picrylhyrazyl (DPPH), while W-CBP50 and W-CBP50I could scavenge DPPH, hydroxyl and superoxide radical. Structural characteristics of W-CBP50 II were investigated using GPC, GC–MS, 1H NMR and IR. The results showed that it was mainly composed of α-glucose, α-mannose, α-galactose and α-arabinose with α-type glycosidic linkage and the molecular weight of its main component was 8.97 kD.

{Author Address}:   http://www.sciencedirect.com

{Language}: English

{Reference Type}: Journal Article

{Author}: .Yang Zhu；Qian Li；Guanghua Mao；Ye Zou；Weiwei Feng；Daheng Zheng；Wei Wang；Lulu Zhou；Tianxiu Zhang；Jun Yang；Liuqing Yang；Xiangyang Wu

{Year}: 2014

{Title}:Optimization of enzyme-assisted extraction and characterization of polysaccharides from Hericium erinaceus

{Tag}: 0

{Star}: 0

{Volume}:101

{Issue}: 0

{Pages}: 606-613

{DOL}: http://dx.doi.org/10.1016/j.carbpol.2013.09.099

{ISBN/ISSN}: 0144-8617

{Keywords}: Response surface methodology；Optimization；Enzyme-assisted extraction；Characterization；Hericium erinaceus polysaccharides

{Abstract}: The enzyme-assisted extraction (EAE) of polysaccharides from the fruits of Hericium erinaceus was studied. In this study, response surface methodology and the Box–Behnken design based on single-factor and orthogonal experiments were applied to optimize the EAE conditions. The optimal extraction conditions were as follows: a pH of 5.71, a temperature of 52.03 °C and a time of 33.79 min. The optimal extraction conditions resulted in the highest H. erinaceus polysaccharides (HEP) yield, with a value 13.46 ± 0.37%, which represented an increase of 67.72% compared to hot water extraction (HWE). The polysaccharides were characterized by FT-IR, SEM, CD, AFM, and GC. The results showed that HEP was composed of mannose, glucose, xylose, and galactose in a molar ratio of 15.16:5.55:4.21:1. The functional groups of the H. erinaceus polysaccharides extracted by HWE and EAE were fundamentally identical but had apparent conformational changes.

{Author Address}:   http://www.sciencedirect.com/science/article/pii/S0144861713010011

{Language}: English