{Reference Type}: Journal Article
{Author}: Gong, Zhenhua; Su, Ying; Huang, Lei; Lin, Juan; Tang, Kexuan; Zhou, Xuanwei
{Year}: 2009
{Title}: Cloning and analysis of glyceraldehyde-3-phosphate dehydrogenase gene from Cordyceps militaris
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF AGRICULTURAL RESEARCH
{Volume}: 4
{Issue}: 4
{Pages}: 402-408
{ISBN/ISSN}: 1991-637X
{Keywords}: ASYMMETRIC INTERLACED PCR; HYGROMYCIN-B RESISTANCE; SCHIZOPHYLLUM-COMMUNE; ASPERGILLUS-NIDULANS; FILAMENTOUS FUNGI; PROMOTER; EXPRESSION; SEQUENCES; YEAST; TRANSFORMATION; Glyceraldehyde-3-phosphate dehydrogenase; Cordyceps militaris; TAIL-PCR; promoter
{Abstract}: A gene encoding a glyceraldehyde-3-phosphate dehydrogenase (GPD) gene was isolated from Cordyceps militaris using degenerate PCR and Thermal Asymmetric Interlaced PCR (TAIL-PCR) technology. Analysis of 4493 bp segments (Cmgpd) revealed the cloned gene contains a 2515 bp 5' upstream region, a 1296 bp coding region and a 682 bp 3' downstream region. The coding region contains a 279 bp intron. After cutting the intron, the open reading frame (ORF) with 1017 bp encodes a polypeptide of 338 amino acid residues. The deduced amino acid sequence indicates a proprotein with a molecular weight of 36.18 kDa. There are one TATA box and two possible CAAT boxes lying in the 5' upstream region. The deduced amino acid sequence of C. militaris GPD shared different homology (ranging from 77-94%) with gpd genes from yeast and filamentous fungi species, such as Beauveria bassiana, Gibberella zeae, Myrothecium gramineum. The cloning of the gene not only provides a basis for the further investigation of its structure, expression and regulation mechanism, but also the upstream promoter of Cmgpd has the potential use for directing high and constitutive expression of homologous and heterologous genes.
{Author Address}: Shanghai Jiao Tong Univ, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Sch Agr & Biol, Plant Biotechnol Res Ctr, Shanghai 200240, Peoples R China; Shanghai Jiao Tong Univ, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Sch Agr & Biol, Plant Biotechnol Res Ctr, Shanghai 200240, Peoples R China; Shanghai Jiao Tong Univ, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Sch Agr & Biol, Plant Biotechnol Res Ctr, Shanghai 200240, Peoples R China; Fudan Univ, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China; Shanghai Jiao Tong Univ, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Sch Agr & Biol, Plant Biotechnol Res Ctr, Shanghai 200240, Peoples R China; Shanghai Jiao Tong Univ, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Sch Agr & Biol, Plant Biotechnol Res Ctr, Shanghai 200240, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China; Peoples R China

{Reference Type}: Journal Article
{Author}: Moussa, Tarek A. A.
{Year}: 2011
{Title}: Cloning and sequencing of phenol oxidase 1 (pox1) gene from Pleurotus ostreatus
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{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 10
{Issue}: 8
{Pages}: 1299-1308
{ISBN/ISSN}: 1684-5315
{Keywords}: CU,ZN SUPEROXIDE-DISMUTASE; METAL-RESPONSIVE ELEMENTS; LACCASE GENE; PICHIA-PASTORIS; MOLECULAR-CLONING; PYCNOPORUS-CINNABARINUS; TRAMETES-VERSICOLOR; FUNGAL LACCASES; CERIPORIOPSIS-SUBVERMISPORA; SACCHAROMYCES-CEREVISIAE; Pleurotus; cDNA; pox1; gene promoter; putative sequences; northern blot analysis; copper
{Abstract}: The gene (pox1) encoding a phenol oxidase 1 from Pleurotus ostreatus was sequenced and the corresponding pox1-cDNA was also synthesized, cloned and sequenced. The isolated gene is flanked by an upstream region called the promoter (399 bp) prior to the start codon (ATG). The putative metal-responsive elements (MREs) were determined in the promoter region, where MRE 1, 2 and 3 were located in positions -20, -62 and -389, respectively. Functional TATA consensus sequences were recognized in positions -78 and -245, while CAAT consensus sequence was recognized in position -171. The putative GC boxes consensus sequences were recognized in positions -175 and -344, and xenobiotic-responsive elements (XREs) in positions -100 and -270. The pox1-DNA gene consists of 2656 bp, with the coding sequence being interrupted by 19 introns. The nucleotide sequence of cDNA (pox1-cDNA) was found to contain an ORF of 1590 bp capable of coding for a protein of 529 amino acid residues. The signal peptide was predicted to be 23 amino acids in length using SIGNALP 3.0 program. Northern blot analysis revealed that strong transcriptional induction was observed in the copper-supplemented cultures for pox1 gene.
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Egypt

{Reference Type}: Journal Article
{Author}: Khan, Samiya Mahmood; Nawaz, Aamir; Malik, Waqas; Javed, Nazir; Yasmin, Tahira; Rehman, Mehboob Ur; Qayyum, Abdul; Iqbal, Qumer; Ahmad, Tanveer; Khan, Azhar Ali
{Year}: 2011
{Title}: Morphological and molecular characterization of Oyster mushroom (Pleurotus spp.)
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 10
{Issue}: 14
{Pages}: 2638-2643
{ISBN/ISSN}: 1684-5315
{Keywords}: IMPROVEMENT; PAKISTAN; MARKERS; TAXA; Oyster mushrooms; genetic diversity; random amplified polymorphic DNA (RAPD) markers; dendrogram
{Abstract}: Mushrooms have medicinal as well as nutritive value and extensively used as human food from the time immortal. In order to determine the genetic diversity among Pleurotus species of mushroom using morphological and random amplified polymorphic DNA (RAPD) markers, about seven different species were collected. Five species, naming Pleurotus platypus (P-6), Pleurotus flabelatus (P-7), Pleurotus florida (P-17), Pleurotus ostreatus (P-19) and Pleurotus sajor-caju (P-56) were from Canada and two Pleurotus warm-stram (P-9) and Pleurotus eryngii (P-16) from Philpines. Seven different morphological traits that is, mycelial growth (mm), cap diameter (cm), total yield (kg), moisture contents (%), ash contents (%), nitrogen contents (%) and protein content (%) were recorded. The dendrogram based on morphological data divided seven species in cluster 'A' and 'B' having four and three species, respectively. The dendrogram based on RAPD analysis generated 3 clusters 'A', 'B' and 'C'. Out of 14 random primers, the maximum polymorphism was observed by primers OPL(3) (72.70 %) and OPL(11) (70%). The two species P-56 and P-17 were observed to be most similar having value 86% and constituting a cluster 'A'. The present work revealed that, morphological and RAPD markers showed different pattern of genetic diversity among different Pleurotus species.
{Author Address}: Bahauddin Zakariya Univ, Dept Plant Pathol, Univ Coll Agr, Multan, Pakistan; Bahauddin Zakariya Univ, Dept Hort, Univ Coll Agr, Multan, Pakistan; Bahauddin Zakariya Univ, Dept Genet & Plant Breeding, Univ Coll Agr, Multan, Pakistan; Univ Agr Faisalabad, Dept Plant Pathol, Faisalabad, Pakistan; Univ Agr Faisalabad, Dept Bot, Faisalabad, Pakistan; NIBGE, Faisalabad, Pakistan; Bahauddin Zakariya Univ, Dept Genet & Plant Breeding, Univ Coll Agr, Multan, Pakistan; Univ Agr Faisalabad, Inst Hort Sci, Faisalabad, Pakistan; Univ Agr Faisalabad, Inst Hort Sci, Faisalabad, Pakistan; PARC Substn, Multan, Pakistan
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Pakistan; Pakistan; Pakistan; Pakistan; Pakistan; Pakistan; Pakistan; Pakistan

{Reference Type}: Journal Article
{Author}: Lei, Wei; Chen, Hai; Zhang, Gu-ren; Li, Shao-song; Peng, Qing-yun; Zhong, Xin; Liu, Xin
{Year}: 2011
{Title}: Molecular identification and food source inference of constructive plants, native to the Ophiocordyceps sinensis habitat
{Tag}: 0
{Star}: 0
{Journal}: AFRICAN JOURNAL OF BIOTECHNOLOGY
{Volume}: 10
{Issue}: 2
{Pages}: 159-167
{ISBN/ISSN}: 1684-5315
{Keywords}: Phylogenetic inference; molecular identification; constructive plant; Ophiocordyceps sinensis; Hepialus pui
{Abstract}: Ophiocordyceps sinensis is a precious and effectual Traditional Chinese Medicine, native to Tibetan Plateau. Its development originated from parasitization of Hirsutella sinensis to Hepialus larvae, which was deemed to be of great importance including feeding food habit. Hereby in the present, study on food resource plants and their molecular identification technique were performed by some methods such as vegetation investigation, morphological taxonomy, sequence alignment, phylogenetic inference, primer design and correlation analysis and so on. In consequence, molecular identification system of constructive plants native to the O. sinensis habitat was established, and then feeding food habit of Hepialus pui larvae and its correlation with genesis mechanism of O. sinensis were preliminarily analyzed and discussed. It is very significant for phylogenetic systematicbotany research of plateau plants on a relative large scale, and it is the first time to study feeding food habit of Hepialus larvae and its related infected process on the molecular level.
{Author Address}: Sun Yat Sen Univ, Food & Hlth Engn Res Ctr, State Educ Minist, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China; Sun Yat Sen Univ, Food & Hlth Engn Res Ctr, State Educ Minist, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China; Sun Yat Sen Univ, Food & Hlth Engn Res Ctr, State Educ Minist, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China; Sun Yat Sen Univ, Food & Hlth Engn Res Ctr, State Educ Minist, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China; Sun Yat Sen Univ, Food & Hlth Engn Res Ctr, State Educ Minist, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China; Sun Yat Sen Univ, Food & Hlth Engn Res Ctr, State Educ Minist, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China; Sun Yat Sen Univ, Food & Hlth Engn Res Ctr, State Educ Minist, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
{Database Provider}: Web of Science SCI
{Language}: English
{Country}: Peoples R China